DNA fingerprinting of Riemerella anatipestifer

被引：17

作者：

Rimler, RB ^{[1
]}

Nordholm, GE ^{[1
]}

机构：

[1] USDA ARS, Natl Anim Dis Ctr, Avian & Swine Resp Dis Res Unit, Ames, IA 50010 USA

来源：

AVIAN DISEASES | 1998年 / 42卷 / 01期

关键词：

DNA fingerprinting; restriction endonuclease analysis; Riemerella anatipestifer; serology; endonuclease; epidemiology;

D O I：

10.2307/1592581

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Seventeen restriction endonucleases were evaluated for use in DNA fingerprinting of Riemerella anatispestifer. Digestion of chromosomal DNA with either HinfI or DdeI restriction endonuclease, followed by submarine electrophoresis in agarose and staining with ethidium bromide, resulted in DNA fingerprint profiles that could be easily resolved. HinfI produced readable fingerprint patterns in the 2.7-20-kb range and was used to distinguish DNA fingerprint profiles among 89 strains of R. anatipestifer representing isolates from various avian species in the United States, the United Kingdom, Australia, Canada, Germany, and Israel. A total of 52 distinct DNA fingerprint profiles were found.

引用

页码：101 / 105

页数：5

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