Quantitation of rabbit cytokine mRNA by real-time RT-PCR

被引：103

作者：

Godornes, Charmie

Leader, Brandon Troy

Molini, Barbara J.

Centurion-Lara, Arturo

Lukehart, Sheila A.

机构：

[1] Univ Washington, Dept Med, Harborview Med Ctr, Seattle, WA 98104 USA

[2] Univ Washington, Dept Pathobiol, Seattle, WA 98104 USA

来源：

CYTOKINE | 2007年 / 38卷 / 01期

关键词：

rabbit; cytokines; quantitative real-time RT-PCR; syphilis;

D O I：

10.1016/j.cyto.2007.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fundamental understanding of rabbit immunology and the use of the rabbit as a disease model have long been hindered by the lack of immunological assays specific to this species. In the present study, we sought to develop a method to quantitate cytokine expression in rabbit cells and tissues. We report the development of a quantitative real-time RT-PCR method for measuring the relative levels of rabbit IFN-gamma IL-2, IL-4 IL-10 and TNF-alpha mRNA. Quantitation was accomplished by comparison to a standard curve generated using plasmid DNA containing partial sequences of the relevant cytokines. Experimental studies demonstrate applicability of this assay to quantitate cytokine mRNA levels from rabbit spleen cells following mitogen stimulation. We have further utilized this assay to also examine cytokine expression in rabbit tissues during experimental syphilis infection. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1 / 7

页数：7

共 26 条

[1] Kinetics of cytokine gene expression in human CD4+ and CD8+ T-lymphocyte subsets using quantitative real-time PCR [J].