Matrix metalloproteinase-1 expression by interaction between monocytes and vascular endothelial cells

被引:46
作者
Hojo, Y
Ikeda, U [1 ]
Takahashi, M
Sakata, Y
Takizawa, T
Okada, K
Saito, T
Shimada, K
机构
[1] Jichi Med Sch, Dept Cardiol, Minami Kawachi, Tochigi 3290498, Japan
[2] Jichi Med Sch, Dept Thrombosis & Hemostasis, Minami Kawachi, Tochigi 3290498, Japan
[3] Jichi Med Sch, Dept Anat, Minami Kawachi, Tochigi 3290498, Japan
[4] Jichi Med Sch, Dept Endocrinol & Metab, Minami Kawachi, Tochigi 3290498, Japan
关键词
atherosclerosis; extracellular matrix; cytokine; remodeling; protein kinase;
D O I
10.1006/jmcc.2000.1179
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
There is accumulating evidence of complicated interactions among vascular cells, i.e. endothelial cells, smooth muscle cells and monocytes/macrophages, in the regulation of vascular function and remodeling. We have investigated the mechanisms responsible for matrix metalloproteinase (MMP)-1 expression by interactions between monocytes and vascular endothelial cells. THP-1 cells (human monocytic cell line) and human umbilical vein endothelial cells (HUVECs) were cocultured. MMP-1 levels in the culture medium were measured by enzyme-limited immunosorbent assays. Collagenolytic activity in the culture medium was measured by fluorescence labeled-collagen digestion. Immunohistochemistry using an anti-MMP antibody was carried out to determine which types of cell produce MMP-1. The addition of THP-1 cells to HUVECs for 48 h induced increases in MMP-1 levels and collagenolytic activity, which were 5- and 2-fold relative to those of HUVECs alone, respectively. A separate coculture experiment revealed that direct contact of THP-1 cells and HUVECs contributed to enhanced MMP-1 production in the cocolture. Immunohistochemical analysis revealed that both types of cell produce MMP-1 in the coculture. Neutralizing anti-interleukin-1 beta and tumor necrosis factor-alpha antibodies inhibited MMP-1 production by the coculture, The Src kinase and MEK inhibitors significantly inhibited MMP-1 production by the coculture. Coculture of THP-1 cells and HUVECs induced significant increases in Src and mitogen activated protein (MAP) kinase activities. Enhanced MMP-1 expression induced by monocyte-endothelial cell interactions may play an important role in the pathogenesis of atherosclerosis and plaque rupture. (C) 2000 Academic Press.
引用
收藏
页码:1459 / 1468
页数:10
相关论文
共 41 条
  • [1] ANDJIT F, 1998, J IMMUNOL, V160, P2967
  • [2] [Anonymous], 1997, Cardiovasc Res, V35, P2
  • [3] CHANGES IN IGG FC RECEPTOR EXPRESSION INDUCED BY PHORBOL 12-MYRISTATE 13-ACETATE TREATMENT OF THP-1 MONOCYTIC LEUKEMIA-CELLS
    AUWERX, J
    STAELS, B
    VANVAECK, F
    CEUPPENS, JL
    [J]. LEUKEMIA RESEARCH, 1992, 16 (03) : 317 - &
  • [4] DISTRIBUTION OF CIRCUMFERENTIAL STRESS IN RUPTURED AND STABLE ATHEROSCLEROTIC LESIONS - A STRUCTURAL-ANALYSIS WITH HISTOPATHOLOGICAL CORRELATION
    CHENG, GC
    LOREE, HM
    KAMM, RD
    FISHBEIN, MC
    LEE, RT
    [J]. CIRCULATION, 1993, 87 (04) : 1179 - 1187
  • [5] DAVIES MJ, 1993, BRIT HEART J, V69, P377
  • [6] Foda HD, 1996, LAB INVEST, V74, P538
  • [7] Monocytes modulate the fibrinolytic balance of endothelial cells
    Funayama, H
    Sakata, Y
    Kitagawa, S
    Ikeda, U
    Takahashi, M
    Masuyama, J
    Mimuro, J
    Matsuda, M
    Shimada, K
    [J]. THROMBOSIS RESEARCH, 1997, 85 (05) : 377 - 385
  • [8] CYTOKINE-STIMULATED HUMAN VASCULAR SMOOTH-MUSCLE CELLS SYNTHESIZE A COMPLEMENT OF ENZYMES REQUIRED FOR EXTRACELLULAR-MATRIX DIGESTION
    GALIS, ZS
    MUSZYNSKI, M
    SUKHOVA, GK
    SIMONMORRISSEY, E
    UNEMORI, EN
    LARK, MW
    AMENTO, E
    LIBBY, P
    [J]. CIRCULATION RESEARCH, 1994, 75 (01) : 181 - 189
  • [9] MACROPHAGE FOAM CELLS FROM EXPERIMENTAL ATHEROMA CONSTITUTIVELY PRODUCE MATRIX-DEGRADING PROTEINASES
    GALIS, ZS
    SUKHOVA, GK
    KRANZHOFER, R
    CLARK, S
    LIBBY, P
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (02) : 402 - 406
  • [10] GALLIS ZS, 1994, J CLIN INVEST, V94, P2493