Graphite-teflon-peroxidase composite electrodes. Application to the direct determination of glucose in musts and wines

被引:44
作者
del Cerro, MA [1 ]
Cayuela, G
Reviejo, AJ
Pingarron, JM
Wang, J
机构
[1] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, E-28040 Madrid, Spain
[2] New Mexico State Univ, Dept Chem, Las Cruces, NM 88003 USA
关键词
graphite-teflon composite enzyme electrodes; peroxidase; glucose;
D O I
10.1002/elan.1140091415
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The performance of graphite-70% Teflon-peroxidase-ferrocene composite electrodes, fabricated by simple physical inclusion of the enzyme and the mediator into the bulk of a graphite-Teflon matrix, by using both batch and flow-injection modes, is reported. The electrode responds very rapidly to changes in H2O2 concentration due to the absence of a membrane barrier on the electrode surface and of a covalent attachment of the enzyme to graphite. The composite enzyme electrode showed a long-term operation due to the renewability of its surface by polishing. Reproducible amperometric responses were achieved with different electrodes fabricated from different composite matrices, and no significant loss of the enzyme activity was observed after seven months of dry-storage at 4 degrees C. A bienzymatic graphite-70% Teflon-peroxidase-glucose oxidase-ferrocene electrode has been developed, too, showing a rapid response to the changes in the glucose concentration. The stability of the bienzymatic composite electrode was similar to that of the peroxidase biosensor. This biosensor was applied to the determination of glucose in must and wine samples by batch amperometry and dow injection with amperometric detection modes.
引用
收藏
页码:1113 / 1119
页数:7
相关论文
共 16 条
  • [1] Alegret S, 1996, SENSOR MATER, V8, P147
  • [2] THIN-FILM BIENZYME AMPEROMETRIC BIOSENSORS BASED ON POLYMERIC REDOX MEDIATORS WITH ELECTROSTATIC BIPOLAR PROTECTING LAYER
    BOGUSLAVSKY, L
    KALASH, H
    XU, Z
    BECKLES, D
    GENG, L
    SKOTHEIM, T
    LAURINAVICIUS, V
    LEE, HS
    [J]. ANALYTICA CHIMICA ACTA, 1995, 311 (01) : 15 - 21
  • [3] BIOCHEMICAL ASPECTS OF BIOSENSORS
    BYFIELD, MP
    ABUKNESHA, RA
    [J]. BIOSENSORS & BIOELECTRONICS, 1994, 9 (4-5) : 373 - 400
  • [4] EISENTHAL R, 1992, ENZYME ASSAYS, P284
  • [5] CARBON-PASTE ELECTRODES MODIFIED WITH ENZYMES, TISSUES, AND CELLS
    GORTON, L
    [J]. ELECTROANALYSIS, 1995, 7 (01) : 23 - 45
  • [6] AMPEROMETRIC BIOSENSORS BASED ON AN APPARENT DIRECT ELECTRON-TRANSFER BETWEEN ELECTRODES AND IMMOBILIZED PEROXIDASES - PLENARY LECTURE
    GORTON, L
    JONSSONPETTERSSON, G
    CSOREGI, E
    JOHANSSON, K
    DOMINGUEZ, E
    MARKOVARGA, G
    [J]. ANALYST, 1992, 117 (08) : 1235 - 1241
  • [7] HART FL, 1991, ANAL MODERNO ALIMENT, P38
  • [8] DIFFERENTIAL PULSE POLAROGRAPHIC-DETERMINATION OF SOME CARCINOGENIC NITROSAMINES
    HASEBE, K
    OSTERYOUNG, J
    [J]. ANALYTICAL CHEMISTRY, 1975, 47 (14) : 2412 - 2418
  • [9] SENSORS BASED ON CARBON-PASTE IN ELECTROCHEMICAL ANALYSIS - A REVIEW WITH PARTICULAR EMPHASIS ON THE PERIOD 1990-1993
    KALCHER, K
    KAUFFMANN, JM
    WANG, J
    SVANCARA, I
    VYTRAS, K
    NEUHOLD, C
    YANG, Z
    [J]. ELECTROANALYSIS, 1995, 7 (01) : 5 - 22
  • [10] PRINCIPLES OF ENVIRONMENTAL-ANALYSIS
    KEITH, LH
    CRUMMETT, W
    DEEGAN, J
    LIBBY, RA
    TAYLOR, JK
    WENTLER, G
    [J]. ANALYTICAL CHEMISTRY, 1983, 55 (14) : 2210 - 2218