Application of Proteomic Profiling Based on 2D-DIGE for Classification of Compounds According to the Mechanism of Action

被引：66

作者：

Muroi, Makoto ^{[1
]}

Kazami, Sayaka ^{[1
,2
]}

Noda, Kazue ^{[1
]}

Kondo, Hisae ^{[1
]}

Takayama, Hiroshi ^{[1
,2
]}

Kawatani, Makoto ^{[1
]}

Usui, Takeo ^{[3
]}

Osada, Hiroyuki ^{[1
,2
]}

机构：

[1] RIKEN Adv Sci Inst, Dept Biol Chem, Chem Biol Core Facil, Chem Lib Validat Team, Wako, Saitama 3510198, Japan

[2] Saitama Univ, Grad Sch Sci & Engn, Saitama 3388570, Japan

[3] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan

来源：

CHEMISTRY & BIOLOGY | 2010年 / 17卷 / 05期

关键词：

DNA TOPOISOMERASE-II; TUBULIN POLYMERIZATION; ANTITUMOR-ACTIVITY; SMALL MOLECULES; INHIBITORS; TARGET; IDENTIFICATION; ANTIBIOTICS; EXPRESSION; PHALLOIDIN;

D O I：

10.1016/j.chembiol.2010.03.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of new anticancer agents derived from natural resources requires a rapid identification of their molecular mechanism of action. To make this step short, we have initiated the proteomic profiling of He La cells treated with anticancer drugs representing a wide spectrum of mechanisms of action using two-dimensional difference gel electrophoresis (2D-DIGE). Unique proteome patterns were observed in He La cells treated with the HSP90 inhibitor geldanamycin, and were similar to the patterns induced by radicicol, a structurally different HSP90 inhibitor. On the other hand, etoposide and ICRF-193, compounds claimed to be topoisomerase II inhibitors, showed different proteomic profiles, which reflect their different biological activities as revealed by cell-cycle analysis. Thus far, combined data from 19 compounds have allowed their successful classification by cluster analysis according to the mechanism of action.

引用

页码：460 / 470

页数：11

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