Improvement of biosensor performances for nitrate determination using a new hydrophilic poly (pyrrole-viologen) film

被引：61

作者：

Da Silva, S ^{[1
]}

Shan, D ^{[1
]}

Cosnier, S ^{[1
]}

机构：

[1] Univ Grenoble 1, Inst Chim Mol Grenoble, CNRS, UMR 5630,Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble 9, France

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2004年 / 103卷 / 1-2期

关键词：

biosensor; polypyrrole; nitrate reductase; viologen;

D O I：

10.1016/j.snb.2004.04.068

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemical biosensors for nitrate determination are developed based on the immobilization of nitrate reductase (NR) within two different redox matrixes, one being a pure organic polymer and the other a clay-polypyrrole composite. NR is co-adsorbed with a poorly soluble pyrrole-viologen on an electrode surface and immobilized by physical entrapment during the subsequent electropolymerization of the adsorbed monomers (bioelectrode 1). A new bioelectrode design for the immobilization and electrical wiring of NR is based on the NR entrapment in a laponite clay gel acting as an inorganic template for the electropolymerization of a new water-soluble pyrrole-viologen (bioelectrode 2). The resulting biosensors performances for nitrate determination are investigated by cyclic voltammetry. In contrast with bioelectrode 1, bioelectrode 2 exhibits a marked catalytic response in presence of nitrates. The apparent Michaelis-Menten constant is 0.21 mM. The sensitivity and detection limit of the bioelectrode 2 for nitrate are 94.7 mA M-1 cm(-2) and 0.5 muM, respectively. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：397 / 402

页数：6

共 29 条

[1] Improvement of the analytical characteristics of an enzyme electrode for free and total cholesterol via laponite clay additives [J].