Identification of disulfide bonds in the ninth component (C9) of human complement

被引：17

作者：

Lengweiler, S ^{[1
]}

Schaller, J ^{[1
]}

Rickli, EE ^{[1
]}

机构：

[1] UNIV BERN,INST BIOCHEM,CH-3012 BERN,SWITZERLAND

来源：

FEBS LETTERS | 1996年 / 380卷 / 1-2期

关键词：

complement; C9; disulfide bridge; terminal complement component;

D O I：

10.1016/0014-5793(95)01541-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C9 is the most abundant protein of the membrane attack complex of complement, By means of limited proteolysis, different chromatographic techniques, a thiol-specific fluorescence assay, amino acid analysis, and Edman degradation, 9 out of 12 disulfide bridges are definitely assigned (Cys(22)-Cys(57), Cys(33)-Cys(36), Cys(67)-Cys(73), Cys(121)-Cys(160), Cys(233)-Cys(234), Cys(359)-Cys(384), Cys(489)-Cys(505), Cys(492)-Cys(507), Cys(509)-Cys(518)), Weaker evidence permits to reduce the number of possible configurations for the remaining 3 cystines (Cys(80)-Cys(91), Cys(86)-Cys(104), Cys(98)-Cys(113), or Cys(80)-Cys(91), Cys(86)-Cys(113), Cys(98)-Cys(104)). These findings are disussed in comparison with the strongly related components C6, C7, C8 alpha, and C8 beta.

引用

页码：8 / 12

页数：5

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