Acquisition and diffusion of blaCTX-M-9 gene by R478-IncHI2 derivative plasmids

被引：48

作者：

Garcia, Aurora

Navarro, Ferran

Miro, Elisenda

Villa, Laura

Mirelis, Beatriz

Coll, Pere

Carattoli, Alessandra

机构：

[1] Hosp Santa Creu & Sant Pau, Microbiol Serv, Barcelona 08025, Spain

[2] Univ Autonoma Barcelona, Dept Genet & Microbiol, Unitat Microbiol, Bellaterra, Spain

[3] Ist Super Sanita, Dept Infect Parasit & Immune Mediated, Rome, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 271卷 / 01期

关键词：

ESBL; mobile elements; mechanisms of resistance; antimicrobial resistance;

D O I：

10.1111/j.1574-6968.2007.00695.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Escherichia coli and Salmonella enterica isolates carrying the bla(CTX-M-9) gene located on plasmids prevailed at the Hospital de la Santa Creu i Sant Pau, Barcelona, Spain in the 1996-1999 period. The bla(CTX-M-9)-carrying plasmids showed a great variability in size, suggesting the mobilization of the gene among different plasmid scaffolds. The aim of the present work was to identify and better characterize the plasmids involved in the spread of the bla(CTX-M-9) gene. Results showed that the majority of these strains carried plasmids belonging to the IncHI2 incompatibility group. The IncHI2 plasmids were further characterized, and found to be related to the reference IncHI2 plasmid R478.

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收藏

页码：71 / 77

页数：7

相关论文

共 19 条

[1] A GENERAL-METHOD FOR DETECTING AND SIZING LARGE PLASMIDS [J].

BARTON, BM ;

HARDING, GP ;

ZUCCARELLI, AJ .

ANALYTICAL BIOCHEMISTRY, 1995, 226 (02) :235-240

[2] Growing group of extended-spectrum β-lactamases:: The CTX-M enzymes [J].

Bonnet, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (01) :1-14

[3] Extended-spectrum β-lactamases in the 21st century:: Characterization, epidemiology, and detection of this important resistance threat [J].

Bradford, PA .

CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (04) :933-951

[4] Identification of plasmids by PCR-based replicon typing [J].

Carattoli, A ;

Bertini, A ;

Villa, L ;

Falbo, V ;

Hopkins, KL ;

Threlfall, EJ .

JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 63 (03) :219-228

[5] IDENTIFICATION AND CLASSIFICATION OF BACTERIAL PLASMIDS [J].

COUTURIER, M ;

BEX, F ;

BERGQUIST, PL ;

MAAS, WK .

MICROBIOLOGICAL REVIEWS, 1988, 52 (03) :375-395

[6] Characterization of the highly variable region surrounding the blaCTX-M-9 gene in non-related Escherichia coli from Barcelona [J].

García, A ;

Navarro, F ;

Miró, E ;

Mirelis, B ;

Campoy, S ;

Coll, P .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 56 (05) :819-826

[7] The complete nucleotide sequence of the resistance plasmid R478: defining the backbone components of incompatibility group H conjugative plasmids through comparative genomics [J].

Gilmour, MW ;

Thomson, NR ;

Sanders, M ;

Parkhill, J ;

Taylor, DE .

PLASMID, 2004, 52 (03) :182-202

[8] Diversity of silver resistance genes in IncH incompatibility group plasmids [J].

Gupta, A ;

Phung, LT ;

Taylor, DE ;

Silver, S .

MICROBIOLOGY-SGM, 2001, 147 :3393-3402

[9] R-FACTORS FROM SERRATIA-MARCESCENS [J].

HEDGES, RW ;

RODRIGUEZLEMOIN.V ;

DATTA, N .

JOURNAL OF GENERAL MICROBIOLOGY, 1975, 86 (JAN) :88-92

[10] Complete DNA sequence comparative genomics, and prevalence of an IncHI2 plasmid occurring among extraintestinal pathogenic Escherichia coli isolates [J].

Johnson, Timothy J. ;

Wannemeuhler, Yvonne M. ;

Scaccianoce, Jennifer A. ;

Johnson, Sara J. ;

Nolan, Lisa K. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (11) :3929-3933