Lotus japonicus contains two distinct ENOD40 genes that are expressed in symbiotic, nonsymbiotic, and embryonic tissues

被引:49
作者
Flemetakis, E
Kavroulakis, N
Quaedvlieg, NEM
Spaink, HP
Dimou, M
Roussis, A
Katinakis, P
机构
[1] Agr Univ Athens, Dept Agr Biotechnol, Athens 11855, Greece
[2] Leiden State Univ, Clusius Lab, Inst Mol Plant Sci, NL-2333 AL Leiden, Netherlands
关键词
nodule organogenesis; symbiosis;
D O I
10.1094/MPMI.2000.13.9.987
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ENOD40, an early nodulin gene, has been postulated to play a significant role in legume root nodule ontogenesis, We have isolated two distinct ENOD40 genes from Lotus japonicus, The transcribed regions of the two ENOD40 genes share 65% homology, while the two promoters showed no significant homology, Both transcripts encode a putative dodecapeptide similar to that identified in other legumes forming determinate nodules, Both ENOD40 genes are coordinately expressed following inoculation of roots with Mesorhizobium loti or treatment with purified Nod factors. In the former case, mRNA accumulation could be detected up to 10 days following inoculation while in the latter case the accumulation was transient. High levels of both ENOD40 gene transcripts were found in nonsymbiotic tissues such as stems, fully developed flowers, green seed pods, and hypocotyls, A relatively lower level of both transcripts was observed in leaves, roots, and cotyledons. In situ hybridization studies revealed that, in mature nodules, transcripts of both ENOD40 genes accumulate in the nodule vascular system; additionally, in young seed pods strong signal is observed in the ovule, particularly In the phloem and epithelium, as well as in globular stage embryos.
引用
收藏
页码:987 / 994
页数:8
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