Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray

被引:98
作者
Takahashi, S
Seki, M
Ishida, J
Satou, M
Sakurai, T
Narusaka, M
Kamiya, A
Nakajima, M
Enju, A
Akiyama, K
Yamaguchi-Shinozaki, K
Shinozaki, K
机构
[1] RIKEN, Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan
[2] RIKEN, Yokohama Inst, Plant Mutat Explorat Team, Plant Funct Genom Res Grp,Genom Sci Ctr, Yokohama, Kanagawa 2300045, Japan
[3] RIKEN, Genom Sci Ctr, Genom Knowledge Base Res Team, Bioinformat Grp,Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[4] Minist Agr Forestry & Fisheries, Biol Resources Div, Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3050851, Japan
基金
日本科学技术振兴机构;
关键词
abiotic stress; Arabidopsis thaliana; cDNA microarray; full-length cDNA; T87 cell culture;
D O I
10.1007/s11103-004-2200-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 muM ABA and 10 mM H2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 muM ABA and 10 mM H2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA- dependent signaling pathways.
引用
收藏
页码:29 / 55
页数:27
相关论文
共 73 条
[1]   Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling [J].
Abe, H ;
Urao, T ;
Ito, T ;
Seki, M ;
Shinozaki, K ;
Yamaguchi-Shinozaki, K .
PLANT CELL, 2003, 15 (01) :63-78
[2]   Role of Arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression [J].
Abe, H ;
YamaguchiShinozaki, K ;
Urao, T ;
Iwasaki, T ;
Hosokawa, D ;
Shinozaki, K .
PLANT CELL, 1997, 9 (10) :1859-1868
[3]   DNA microarrays for functional plant genomics [J].
Aharoni, A ;
Vorst, O .
PLANT MOLECULAR BIOLOGY, 2002, 48 (1-2) :99-118
[4]   The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases [J].
Albrecht, V ;
Ritz, O ;
Linder, S ;
Harter, K ;
Kudla, J .
EMBO JOURNAL, 2001, 20 (05) :1051-1063
[5]  
AXELOS M, 1992, PLANT PHYSIOL BIOCH, V30, P123
[6]  
BAZET N, 1998, PLANT J, V13, P519
[7]   ADAPTATIONS TO ENVIRONMENTAL STRESSES [J].
BOHNERT, HJ ;
NELSON, DE ;
JENSEN, RG .
PLANT CELL, 1995, 7 (07) :1099-1111
[8]   Genetic analysis of ABA signal transduction pathways [J].
Bonetta, D ;
McCourt, P .
TRENDS IN PLANT SCIENCE, 1998, 3 (06) :231-235
[9]   Plant responses to water deficit [J].
Bray, EA .
TRENDS IN PLANT SCIENCE, 1997, 2 (02) :48-54
[10]   The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites [J].
Chen, WQ ;
Chao, G ;
Singh, KB .
PLANT JOURNAL, 1996, 10 (06) :955-966