Development of an enzyme immunoassay for the detection of plant growth regulator inabenfide in rice

被引：34

作者：

Watanabe, E

Tsuda, Y

Watanabe, S

Ito, S

Hayashi, M

Watanabe, T

Yuasa, Y

Nakazawa, H

机构：

[1] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan

[2] Environm Immunochem Technol Co Ltd, Satte Off, Satte, Saitama 3400193, Japan

[3] Environm Immunochem Technol Co Ltd, Shizuoka Off, Shizuoka 4390031, Japan

[4] Environm Immunochem Technol Co Ltd, Head Off, Minato Ku, Tokyo 1050013, Japan

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 424卷 / 02期

关键词：

inabenfide; ELISA; rice;

D O I：

10.1016/S0003-2670(00)01076-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A competitive enzyme-linked immunosorbent assay (ELISA) for the plant growth regulator inabenfide was developed using a specific monoclonal antibody (MAB). The effects of rice matrices on the sensibility of ELISA can be removed by adding 0.1% Tween 20. A good reproducibility and accuracy of the proposed ELISA was obtained for rice samples spiked with different amounts of inabenfide. The average recovery for rice samples spiked at 5-500 ppb was 91.6%. Good correlations were obtained between inabenfide measured in rice samples by using the proposed ELISA and that by using a conventional HPLC or GC analysis. This assay can be used in monitoring to determine inabenfide in rice. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：149 / 160

页数：12

共 31 条

[1] Development of an enzyme-linked immunosorbent assay to carbaryl .2. Assay optimization and application to the analysis of water samples [J].