Expression of ORF A1 of infectious bursal disease virus results in the formation of virus-like particles

被引：61

作者：

Fernández-Arias, A

Risco, C

Martínez, S

Albar, JP

Rodríguez, JF

机构：

[1] Ctr Nacl Biotecnol, Dept Biol Mol & Celular, E-28049 Madrid, Spain

[2] Ctr Nacl Biotecnol, Dept Estructura Macromol, E-28049 Madrid, Spain

[3] Ctr Nacl Biotecnol, Dept Immunol & Oncol, E-28049 Madrid, Spain

[4] Ctr Nacl Sanidad Agropecuaria, La Habana, Cuba

来源：

JOURNAL OF GENERAL VIROLOGY | 1998年 / 79卷

关键词：

D O I：

10.1099/0022-1317-79-5-1047

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant vaccinia virus inducibly expressing ORF Al of infectious bursal disease virus (IBDV) has been constructed and characterized. Cells infected with this recombinant virus express the IBDV polyprotein, which is proteolytically processed to give mature VP2, VP3, and VP4 polypeptides. An electron microscopy study revealed that the cytoplasm of cells infected with the recombinant virus contains abundant IBDV-like particles (VLP). These VLP form close-packed paracrystalline arrays that are specifically recognized by anti-IBDV antibodies. The size and morphology of purified VLP were found to be akin to those of authentic IBDV particles.

引用

页码：1047 / 1054

页数：8

共 33 条

[1]

Becht H, 1980, Curr Top Microbiol Immunol, V90, P107

[2]

BOTCHER B, 1997, J VIROL, V71, P325

[3] HERPESVIRUS OF TURKEY RECOMBINANT VIRUSES EXPRESSING INFECTIOUS BURSAL DISEASE VIRUS (IBDV) VP2 IMMUNOGEN INDUCE PROTECTION AGAINST AN IBDV VIRULENT CHALLENGE IN CHICKENS [J].