Huperzine A attenuates the neurotoxic effect of staurosporine in primary rat cortical neurons

被引：26

作者：

Zhang, HY ^{[1
]}

Tang, XC ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2003年 / 340卷 / 02期

基金：

中国国家自然科学基金;

关键词：

huperzine A; cholinesterase inhibitor; primary rat cortical neurons; staurosporine; apoptosis; Bcl-2; Bax; caspase-3;

D O I：

10.1016/S0304-3940(03)00023-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of huperzine A (HupA) on staurosporine-induced neuronal apoptosis and potential mechanisms were investigated in primary cultured rat cortical neurons. Pretreatment of the cells with HupA (0.1-100 muM) 2 h prior to 0.5 muM staurosporine exposure for 24 h, markedly elevated cell survival. Incubation with HupA at higher dose (1 muM) also reduced the DNA fragmentation caused by staurosporine. This dose of HupA also reduced the upregulation of the pro-apoptotic gene, bax, downregulation of the anti-apoptotic gene, bcl-2, and antagonized the decrease in immunoreactive caspase-3 proenzyme. These results demonstrated that HupA protect neurons against staurosporine-induced apoptosis via regulating the apoptotic related genes and caspase-3 proenzyme. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：91 / 94

页数：4

共 17 条

[1] INDUCTION OF A COMMON PATHWAY OF APOPTOSIS BY STAUROSPORINE [J].