Expression of single chain antibodies (ScFvs) for c-myc oncoprotein in recombinant Escherichia coli membranes by using the ice-nucleation protein of Pseudomonas syringae

被引：13

作者：

Bassi, AS ^{[1
]}

Ding, DN

Gloor, GB

Margaritis, A

机构：

[1] Univ Western Ontario, Dept Chem & Biochem Engn, Fac Engn Sci, London, ON N6A 5B9, Canada

[2] Univ Western Ontario, Dept Biochem, London, ON N6A 5B9, Canada

来源：

BIOTECHNOLOGY PROGRESS | 2000年 / 16卷 / 04期

关键词：

D O I：

10.1021/bp000053k

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. In this study, the INP from Pseudomonas syringae was applied as a fusion partner with the single chain antibody fragment (ScFv) against the human oncoprotein c-myc. Two new plasmids pNinaZ-myc and pNinaZScFv-BsaA1 were constructed and cloned into Escherichia coli JM109. The expression of the fusion protein was successfully demonstrated in the cloned cells. The fusion proteins had no effect on the viability of the host cells. Ice nucleation activity measurements and flow cytometry studies were followed to investigate the membrane expression of the fusion protein.

引用

页码：557 / 563

页数：7

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