Enhanced Display of Lipase on the Escherichia coli Cell Surface, Based on Transcriptome Analysis

被引：17

作者：

Baek, Jong Hwan ^{[1
,2
,3
]}

Han, Mee-Jung ^{[1
,2
,3
,6
]}

Lee, Seung Hwan ^{[1
]}

Lee, Sang Yup ^{[1
,2
,3
,4
,5
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Metab & Biomol Engn Natl Res Lab, Bioproc Engn Res Ctr,Program BK21, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Ctr Syst & Synthet Biotechnol, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Inst Biocentury, Taejon 305701, South Korea

[4] Korea Adv Inst Sci & Technol, Dept Biol Sci, Dept Bio & Brain Engn, Taejon 305701, South Korea

[5] Korea Adv Inst Sci & Technol, Bioinformat Res Ctr, Taejon 305701, South Korea

[6] Dongyang Univ, Dept Chem & Biomol Engn, Yeongju 750711, Gyeongbuk, South Korea

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 03期

关键词：

ANCHORING MOTIF; HETEROLOGOUS PROTEINS; FUSION; GENES; OPRF;

D O I：

10.1128/AEM.02463-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis.

引用

页码：971 / 973

页数：3

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