Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays

被引：146

作者：

Fischer, Janine ^{[1
]}

Prosenc, Marc H. ^{[2
]}

Wolff, Martin ^{[3
]}

Hort, Norbert ^{[4
]}

Willumeit, Regine ^{[1
]}

Feyerabend, Frank ^{[1
]}

机构：

[1] GKSS Forschungszentrum Geesthacht GmbH, Dept Struct Res Macromol, Inst Mat Res, D-21502 Geesthacht, Germany

[2] Univ Hamburg, Inst Inorgan & Appl Chem, D-20146 Hamburg, Germany

[3] GKSS Forschungszentrum Geesthacht GmbH, Dept Powder Technol, Inst Mat Res, D-21502 Geesthacht, Germany

[4] GKSS Forschungszentrum Geesthacht GmbH, Magnesium Innovat Ctr, Inst Mat Res, D-21502 Geesthacht, Germany

来源：

ACTA BIOMATERIALIA | 2010年 / 6卷 / 05期

关键词：

Cytotoxicity; Tetrazolium-based assays; MTT; XTT; Magnesium; MTT REDUCTION; CYTO-TOXICITY; FORMAZAN; ALLOYS; ENZYME; CELLS; METAL; BONE; APPROXIMATION; BIOMATERIALS;

D O I：

10.1016/j.actbio.2009.10.020

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Magnesium (Mg) alloys are promising materials for the development of biodegradable implants. However, the current in vitro test procedures for cytotoxicity, cell viability and proliferation are not always suitable for this class of materials. In this paper we show that tetrazolium-salt-based assays, which are widely used in practice, are influenced by the corrosion products of Mg-based alloys. Corroded Mg converts tetrazolium salts to formazan, leading to a higher background and falsifying the results of cell viability. Tetrazolium-based assays are therefore not a useful tool for testing the cytotoxicity of Mg in static in vitro assays. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：1813 / 1823

页数：11

共 36 条

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