Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

被引：28

作者：

Scherl, A

Zimmermann-Ivol, CG

Di Dio, J

Vaezzadehl, AR

Binz, PA

Amez-Droz, M

Cochard, R

Sanchez, JC

Glückmann, M

Hochstrasser, DF

机构：

[1] Univ Hosp Geneva, Biomed Proteom Res Grp, Cent Lab Clin Chem, Geneva, Switzerland

[2] Swiss Inst Bioinformat, Geneva, Switzerland

[3] GeneBio SA, Geneva, Switzerland

[4] Univ Appl Sci, EIG HES SO, Geneva, Switzerland

[5] Appl Biosyst Inc, Darmstadt, Germany

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2005年 / 19卷 / 05期

关键词：

D O I：

10.1002/rcm.1831

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF(TM)) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF(TM) systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry. Copyright (C) 2005 John Wiley Sons, Ltd.

引用

收藏

页码：605 / 610

页数：6

相关论文

共 27 条

[1] Toward a clinical molecular scanner for proteome research: Parallel protein chemical processing before and during western blot [J].

Bienvenut, WV ;

Sanchez, JC ;

Karmime, A ;

Rouge, V ;

Rose, K ;

Binz, PA ;

Hochstrasser, DF .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4800-4807

[2]

Bienvenut WV, 2002, PROTEOMICS, V2, P868, DOI 10.1002/1615-9861(200207)2:7<868::AID-PROT868>3.0.CO

[3]

2-D

[4] A molecular scanner to automate proteomic research and to display proteome images [J].

Binz, PA ;

Müller, M ;

Walther, D ;

Bienvenut, WV ;

Gras, R ;

Hoogland, C ;

Bouchet, G ;

Gasteiger, E ;

Fabbretti, R ;

Gay, S ;

Palagi, P ;

Wilkins, MR ;

Rouge, V ;

Tonella, L ;

Paesano, S ;

Rossellat, G ;

Karmime, A ;

Bairoch, A ;

Sanchez, JC ;

Appel, RD ;

Hochstrasser, DF .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4981-4988

[5] Laser desorption/ionization cluster studies for calibration in mass spectrometry [J].

Blaum, K ;

Huber, G ;

Kluge, HJ ;

Schweikhard, L .

EUROPEAN PHYSICAL JOURNAL D, 2003, 24 (1-3) :145-148

[6] Integrating histology and imaging mass spectrometry [J].

Chaurand, P ;

Schwartz, SA ;

Billheimer, D ;

Xu, BGJ ;

Crecelius, A ;

Caprioli, RM .

ANALYTICAL CHEMISTRY, 2004, 76 (04) :1145-1155

[7] Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry [J].

Chaurand, P ;

Stoeckli, M ;

Caprioli, RM .

ANALYTICAL CHEMISTRY, 1999, 71 (23) :5263-5270

[8] Speciation of gold(III)-L-histidine complex: a multi-instrumental approach [J].

Cuadrado, JA ;

Zhang, WX ;

Hang, W ;

Majidi, V .

JOURNAL OF ENVIRONMENTAL MONITORING, 2000, 2 (04) :355-359

[9] Analysis of proteins by direct scanning infrared-MALDI mass spectrometry after PD PAGE separation and electroblotting [J].

Eckerskorn, C ;

Strupat, K ;

Schleuder, D ;

Hochstrasser, D ;

Sanchez, JC ;

Lottspeich, F ;

Hillenkamp, F .

ANALYTICAL CHEMISTRY, 1997, 69 (15) :2888-2892

[10]

Guittard J, 1999, RAPID COMMUN MASS SP, V13, P1838, DOI 10.1002/(SICI)1097-0231(19990930)13:18<1838::AID-RCM726>3.0.CO