Simultaneous analysis of caffeic acid derivatives and alkamides in roots and extracts of Echinacea purpurea by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry

被引：65

作者：

Luo, XB ^{[1
]}

Chen, B ^{[1
]}

Yao, SZ ^{[1
]}

Zeng, JG ^{[1
]}

机构：

[1] Hunan Phytoway Inc, Changsha 410011, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 986卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Echinacea purpurea; plant materials; caffeic acid; cichoric acid; alkamides;

D O I：

10.1016/S0021-9673(02)01922-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance liquid chromatography (HPLC) coupled with UV photodiode-array detection and electrospray ionization mass spectrometry was developed for the simultaneous analysis of caffeic acid derivatives and alkamides in the roots and extracts of Echinacea purpurea. Caffeic acid derivatives and alkamides produced very abundant peaks in the total ion current chromatogram during negative and positive cone voltage switching. Cichoric acid and the isomer pair, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide, were used as a standard for quantification of caffeic acid derivatives and alkamides in E. purpurea. This novel method surpasses previously published ones in product quality control and providing the HPLC chromatographic fingerprints of biological active components in E. purpurea. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：73 / 81

页数：9

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