学术探索
学术期刊
新闻热点
数据分析
智能评审

Expression of neuronal nitric oxide synthase mRNA in stress-related brain areas after restraint in rats

被引：95
作者：
de Oliveira, RMW
Del Bel, EA
Mamede-Rosa, MLN
Padovan, CM
Deakin, JFW
Guimaraes, FS
机构：
[1] Sch Med, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP, Brazil
[2] Sch Dent, Dept Physiol, BR-14049900 Ribeirao Preto, SP, Brazil
[3] Univ Manchester, Neurosci & Psychiat Unit, Manchester, Lancs, England
来源：
NEUROSCIENCE LETTERS | 2000年 / 289卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
aversion; in situ hybridisation; anxiety; periaqueductal grey; paraventricular nucleus; nitric oxide synthase;
D O I：
10.1016/S0304-3940(00)01287-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The objective of the present study was to investigate the expression of neuronal nitric oxide synthase (nNOS) mRNA in stress-related areas after restraint. Male Wister rats (n = 4-6/group) submitted to 2 h of restraint during one (acute) or seven (chronic) days were sacrificed 24 h after the last restraint period. In situ hybridisation was performed with oligonucleotide probes radiolabeled with S-35. Acute restraint induced a significant increase in nNOS mRNA in the paraventricular hypothalamic nucleus (PVN), medial parvocellular part, dorsolateral periaqueductal grey (DLPAG) and medial amygdaloid nucleus, but not in the hippocampal formation. This effect persisted after chronic restraint in the PVN and DLPAG, These results suggest that restraint stress induces changes in gene expression of nNOS in areas related to stress reactions. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：123 / 126
页数：4
相关论文
共 21 条
[1]  
[Anonymous], HDB ANXIETY
[2]   NITRIC-OXIDE SYNTHASE PROTEIN AND MESSENGER-RNA ARE DISCRETELY LOCALIZED IN NEURONAL POPULATIONS OF THE MAMMALIAN CNS TOGETHER WITH NADPH DIAPHORASE [J].
BREDT, DS ;
GLATT, CE ;
HWANG, PM ;
FOTUHI, M ;
DAWSON, TM ;
SNYDER, SH .
NEURON, 1991, 7 (04) :615-624
[3]   REGIONAL CHANGES IN C-FOS EXPRESSION IN THE BASAL FOREBRAIN AND BRAIN-STEM DURING ADAPTATION TO REPEATED STRESS - CORRELATIONS WITH CARDIOVASCULAR, HYPOTHERMIC AND ENDOCRINE RESPONSES [J].
CHEN, X ;
HERBERT, J .
NEUROSCIENCE, 1995, 64 (03) :675-685
[4]   Behavioral and c-fos expression changes induced by nitric oxide donors microinjected into the dorsal periaqueductal gray [J].
de Oliveira, RW ;
Del Bel, EA ;
Guimaraes, FS .
BRAIN RESEARCH BULLETIN, 2000, 51 (06) :457-464
[5]  
DOURISH CT, 1989, J PSYCHOPHARMACOL, V3, P38
[6]   HIPPOCAMPAL 5-HT RECEPTORS AND CONSOLIDATION OF STRESSFUL MEMORIES [J].
GUIMARAES, FS ;
DELBEL, EA ;
PADOVAN, CM ;
NETTO, SM ;
DEALMEIDA, RT .
BEHAVIOURAL BRAIN RESEARCH, 1993, 58 (1-2) :133-139
[7]   ANXIOLYTIC EFFECT OF NITRIC-OXIDE SYNTHASE INHIBITORS MICROINJECTED INTO THE DORSAL CENTRAL GREY [J].
GUIMARAES, FS ;
DEAGUIAR, JC ;
DELBEL, EA ;
BALLEJO, G .
NEUROREPORT, 1994, 5 (15) :1929-1932
[8]   Immobilization-induced stress activates neuronal nitric oxide synthase (nNOS) mRNA and protein in hypothalamic-pituitary-adrenal axis in rats [J].
Kishimoto, J ;
Tsuchiya, T ;
Emson, PC ;
Nakayama, Y .
BRAIN RESEARCH, 1996, 720 (1-2) :159-171
[9]   LOCALIZATION OF NITRIC-OXIDE SYNTHASE IN THE MOUSE OLFACTORY AND VOMERONASAL SYSTEM - A HISTOCHEMICAL, IMMUNOLOGICAL AND IN-SITU HYBRIDIZATION STUDY [J].
KISHIMOTO, J ;
KEVERNE, EB ;
HARDWICK, J ;
EMSON, PC .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1993, 5 (12) :1684-1694
[10]  
Krukoff TL, 1997, J COMP NEUROL, V377, P509, DOI 10.1002/(SICI)1096-9861(19970127)377:4<509::AID-CNE3>3.0.CO
123