Streptococcus infantarius sp nov., Streptococcus infantarius subsp infantarius subsp nov and Streptococcus infantarius subsp coli subsp nov., isolated from humans and food

被引:73
作者
Schlegel, L
Grimont, F
Collins, MD
Régnault, B
Grimont, PAD
Bouvet, A
机构
[1] Univ Paris 06, Hotel Dieu, Microbiol Serv, Ctr Natl Reference Streptocoques, F-75181 Paris 04, France
[2] Inst Pasteur, INSERM, U389, Unite Enterobacteries, F-75724 Paris, France
[3] Univ Reading, Inst Food Sci & Res, Reading RG6 2AH, Berks, England
关键词
Streptococcus bovis; Streptococcus infantarius; ribotyping; DNA-DNA hybridization; 16S rRNA gene;
D O I
10.1099/00207713-50-4-1425
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Eighteen strains isolated from human specimens or from food products were characterized as atypical variants of mannitol-negative Streptococcus bovis. They were tested for extended biochemical criteria, ribotyping and DNA-DNA hybridization in order to define their taxonomic status. These strains were demonstrated to constitute a DNA relatedness group that includes strains of DNA group 4 of Farrow et al. (1984). Comparative analysis of 16S rRNA sequences demonstrated that these strains represent a new species which belongs to the Streptococcus bovis/Streptococcus equinus complex and which has been provisionally named S. infantarius by Bouvet et al. (1997). Biotyping and ribotyping allowed differentiation of these strains from the aesculin-positive strains of S. bovis belonging to the previously described biotypes I, II.1 and II.2. The results of the ribotyping and hybridization assays demonstrated the presence of two different DNA subgroups within the 18 strains. On the basis of these data, the names S. infantarius subsp. infantarius (aesculin-negative for five strains out of seven, including the type strain HDP 90056(T) = NCDO 599(T)) and 5. infantarius subsp. coli (aesculin-positive, reference strain HDP 90248 = NCDO 2620) are proposed as the names for these two subspecies within the S. infantarius species.
引用
收藏
页码:1425 / 1434
页数:10
相关论文
共 36 条
[1]   DEVELOPMENT OF PCR-BASED HYBRIDIZATION PROTOCOL FOR IDENTIFICATION OF STREPTOCOCCAL SPECIES [J].
BENTLEY, RW ;
LEIGH, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1296-1301
[2]   INTRAGENERIC STRUCTURE OF STREPTOCOCCUS BASED ON COMPARATIVE-ANALYSIS OF SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCES [J].
BENTLEY, RW ;
LEIGH, JA ;
COLLINS, MD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1991, 41 (04) :487-494
[3]  
Bouvet A, 1997, ADV EXP MED BIOL, V418, P393
[4]   INTRASPECIES VARIATIONS IN NUTRITIONALLY VARIANT STREPTOCOCCI - RIBOSOMAL-RNA GENE RESTRICTION PATTERNS OF STREPTOCOCCUS-DEFECTIVUS AND STREPTOCOCCUS-ADJACENS [J].
BOUVET, A ;
GRIMONT, F ;
GRIMONT, PAD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1991, 41 (04) :483-486
[5]   ESCHERICHIA-VULNERIS - A NEW SPECIES OF ENTEROBACTERIACEAE ASSOCIATED WITH HUMAN WOUNDS [J].
BRENNER, DJ ;
MCWHORTER, AC ;
KNUTSON, JKL ;
STEIGERWALT, AG .
JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (06) :1133-1140
[6]  
BRIDGE PD, 1983, J GEN MICROBIOL, V129, P565
[7]   DEOXYRIBONUCLEIC-ACID HYBRIDIZATIONS AMONG STRAINS OF STREPTOCOCCUS-SALIVARIUS AND STREPTOCOCCUS-BOVIS [J].
COYKENDALL, AL ;
GUSTAFSON, KB .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1985, 35 (03) :274-280
[8]   USE OF A SINGLE-STRAND SPECIFIC NUCLEASE FOR ANALYSIS OF BACTERIAL AND PLASMID DEOXYRIBONUCLEIC ACID HOMODUPLEXES AND HETERODUPLEXES [J].
CROSA, JH ;
BRENNER, DJ ;
FALKOW, S .
JOURNAL OF BACTERIOLOGY, 1973, 115 (03) :904-911
[9]  
DOIT C, 1994, PATHOGENIC STREPTOCO, P531
[10]   MEASUREMENT OF ACTIVE CONSTITUTIVE BETA-D-GLUCOSIDASE (ESCULINASE) IN THE PRESENCE OF SODIUM DEOXYCHOLATE [J].
EDBERG, SC ;
TREPETA, RW ;
KONTNICK, CM ;
TORRES, AR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (03) :363-365