Electron-capture mass spectrometry: recent advances

被引:34
作者
Giese, RW
机构
[1] Bouve Coll Pharm & Hlth Profess, Dept Pharmaceut Sci, Barnett Inst, Boston, MA 02115 USA
[2] Northeastern Univ, Dept Chem, Boston, MA 02115 USA
关键词
reviews; electron-capture mass spectrometry; mass spectrometry; instrumentation; bile acids; phenylalanine;
D O I
10.1016/S0021-9673(00)00364-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Electron-capture (EC) is a sensitive and selective ionization technique for mass spectrometry (MS). In the most familiar form of EC, a susceptible analyte (electrophore) is detected after eluting from a gas chromatography (GC) column, where a low attomole detection limit for standards is routine. High-performance liquid chromatography can facilitate sample cleanup prior to detection by GC-EC-MS, but carryover and shifts in retention time for the "invisible" analyte can be difficulties. Solid-phase extraction avoids these difficulties, but the degree of cleanup and recovery can be problems. Alternative electrophoric derivatizing reagents are available to help deal with interferences, and new reagents such as "AMACE1" are emerging. Releasable forms of electrophores can be used as tags for labeling macromolecules, motivated by the desire to multiplex ligand-type assays. The conventional, gas-phase ion source for EC is not well-understood, especially the role of wall reactions. Using an electron monochromator to tune the electron energy adds to the selectivity and information provided by EC-MS. High-resolution and tandem EC-MS measurements are emerging. Electron-capture dissociation is a new technique to sequence small- to medium-sized peptides, having the advantage of providing more extensive sequence information relative to other MS techniques. Particle-beam EC-MS tends to be less sensitive than GC-EC-MS, but not always. Recently it was demonstrated that EC-MS can be accomplished on an ordinary laser desorption time-of-flight mass spectrometer, and also by using atmospheric pressure chemical ionization. Two applications are discussed here in detail: bile acids and oxidized phenylalanine. EC-MS is well-established as a useful technique for trace analysis in special cases, and the scope of its usefulness is broadening (qualitative analysis and detection of more polar and larger molecules), based on advances in both the chemical and instrumental aspects of this technique. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:329 / 346
页数:18
相关论文
共 61 条
[1]   GAS-CHROMATOGRAPHY ELECTRON-CAPTURE NEGATIVE-ION MASS-SPECTROMETRY AT THE ZEPTOMOLE LEVEL [J].
ABDELBAKY, S ;
GIESE, RW .
ANALYTICAL CHEMISTRY, 1991, 63 (24) :2986-2989
[2]   Satellite high-performance liquid chromatography for trace sample cleanup [J].
Abushamaa, A ;
Naim, A ;
Giese, RW .
JOURNAL OF CHROMATOGRAPHY A, 1996, 742 (1-2) :295-297
[3]   DERIVATIZATION IN TRACE ORGANIC-ANALYSIS - SELECTION OF AN INERT SOLVENT [J].
ALLAM, K ;
ABDELBAKY, S ;
GIESE, RW .
ANALYTICAL CHEMISTRY, 1992, 64 (02) :238-239
[4]   KO2 CHEMICAL TRANSFORMATION MASS-SPECTROMETRY DETECTION OF COVALENT DAMAGE TO THE DNA OF CULTURED HUMAN-LYMPHOCYTES EXPOSED TO BENZO[A]PYRENE [J].
ALLAM, K ;
ABDELBAKY, S ;
GIESE, RW .
ANALYTICAL CHEMISTRY, 1993, 65 (13) :1723-1727
[5]   Negative ion laser desorption ionization time-of-flight mass spectrometry of nitrated polycyclic aromatic hydrocarbons [J].
Bezabeh, DZ ;
Allen, TM ;
McCauley, EM ;
Kelly, PB ;
Jones, AD .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1997, 8 (06) :630-636
[6]  
Bian NY, 1997, RAPID COMMUN MASS SP, V11, P1781, DOI 10.1002/(SICI)1097-0231(19971030)11:16<1781::AID-RCM90>3.3.CO
[7]  
2-6
[8]   Trace quantification of the oxidative damage products, meta- and ortho-tryrosine, in biological samples by gas chromatography - Electron capture negative ionization mass spectrometry [J].
Blount, BC ;
Duncan, MW .
ANALYTICAL BIOCHEMISTRY, 1997, 244 (02) :270-276
[9]   Simultaneous determination of multiple aldehydes in biological tissues and fluids using gas chromatography stable isotope dilution mass spectrometry [J].
Bruenner, BA ;
Jones, AD ;
German, JB .
ANALYTICAL BIOCHEMISTRY, 1996, 241 (02) :212-219
[10]  
CAPPIELLO A, 1996, J AM SOC MASS SPECTR, V7, P756