Is in vitro expansion of human cord blood cells clinically relevant?

被引:27
作者
Denning-Kendall, PA
Nicol, A
Horsley, H
Donaldson, C
Bradley, B
Hows, JM
机构
[1] Univ Bristol, Div Transplantat Sci, Bristol, Avon, England
[2] Princess Alexandra Hosp, Woolloongabba, Qld 4102, Australia
关键词
cord blood; CD34(+); colony-forming cells; LTC-IC; expansion;
D O I
10.1038/sj.bmt.1701078
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Peripheral blood recovery after cord blood (CB) transplantation is delayed compared with marrow, Expansion of CB haemopoietic cells has been investigated with the aim of reducing cytopenia following transplantation. Mature cells, post-progenitors, progenitors and longterm culture-initiating cells (LTC-IC) from expansion products may contribute to peripheral blood recovery, We investigated the increase in total nucleated cells, colony-forming cells (CFC), CD34(+) cells and LTC-IC by limiting dilution after a 14 day culture of CB CD34(+) cells (5 x 10(3)/ml) with SCF, IL-3, IL-6, GM-CSF and G-CSF all at 10 ng/ml, On average, nucleated cells increased 2500-fold, CD34(+) cells 39-fold and CFU-GM 49-fold with maintenance of BFU-E, The more primitive LTC-IC expanded on average 2.5-fold but effects on long-term marrow-repopulating cells (LTRC) during culture are unknown, A practical application of in vitro expansion of CB might be to expand a 20% aliquot of a CB donation and infuse the remainder unmanipulated, This could provide a 5- to 7-fold increase in progenitor cells, an estimated 1570-fold increase in post-progenitor cells and maintenance of LTC-IC compared to an untreated donation, Combined with in vivo posttransplant growth factor therapy this could prompt early peripheral blood recovery after CB transplantation, without significant loss of LTC-IC or donor lymphocytes.
引用
收藏
页码:225 / 232
页数:8
相关论文
共 35 条
  • [1] Almici C, 1996, ACTA HAEMATOL-BASEL, V95, P171
  • [2] GROWTH-CHARACTERISTICS AND EXPANSION OF HUMAN UMBILICAL-CORD BLOOD AND ESTIMATION OF ITS POTENTIAL FOR TRANSPLANTATION IN ADULTS
    BROXMEYER, HE
    HANGOC, G
    COOPER, S
    RIBEIRO, RC
    GRAVES, V
    YODER, M
    WAGNER, J
    VADHANRAJ, S
    BENNINGER, L
    RUBINSTEIN, P
    BROUN, ER
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (09) : 4109 - 4113
  • [3] Broxmeyer HE, 1997, CLIN EXP IMMUNOL, V107, P45
  • [4] RETRACTED: RECONSTITUTION OF HEMATOPOIESIS AFTER HIGH-DOSE CHEMOTHERAPY BY AUTOLOGOUS PROGENITOR CELLS GENERATED EX-VIVO (RETRACTED ARTICLE. SEE VOL 345, PG 64, 2001)
    BRUGGER, W
    HEIMFELD, S
    BERENSON, RJ
    MERTELSANN, R
    KANZ, L
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1995, 333 (05) : 283 - 287
  • [5] Cashman J., 1996, Experimental Hematology (Charlottesville), V24, P1035
  • [6] David S, 1995, NOUV REV FR HEMATOL, V37, P343
  • [7] DEBILI N, 1995, BLOOD, V86, P2516
  • [8] DenningKendall P, 1996, EXP HEMATOL, V24, P1394
  • [9] DENNINGKENDALL PA, 1997, BONE MARROW TRANS S1, V19, pS44
  • [10] STIMULATION OF MEGAKARYOCYTOPOIESIS AND THROMBOPOIESIS BY THE C-MPL LIGAND
    DESAUVAGE, FJ
    HASS, PE
    SPENCER, SD
    MALLOY, BE
    GURNEY, AL
    SPENCER, SA
    DARBONNE, WC
    HENZEL, WJ
    WONG, SC
    KUANG, WJ
    OLES, KJ
    HULTGREN, B
    SOLBERG, LA
    GOEDDEL, DV
    EATON, DL
    [J]. NATURE, 1994, 369 (6481) : 533 - 538