Molecular Epidemiology of Pasteurella multocida Circulating in India by Multilocus Sequence Typing

被引：17

作者：

Sarangi, L. N. ^{[1
]}

Thomas, P. ^{[1
]}

Gupta, S. K. ^{[1
]}

Kumar, S. ^{[1
]}

Viswas, K. N. ^{[1
]}

Singh, V. P. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Vet Bacteriol & Mycol, Bareilly 243122, Uttar Pradesh, India

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2016年 / 63卷 / 02期

关键词：

multilocus sequence typing; Pasteurella multocida; India; haemorrhagic septicaemia; epidemiology; HEMORRHAGIC SEPTICEMIA; IDENTIFICATION; OUTBREAK; EBURST;

D O I：

10.1111/tbed.12270

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Multilocus sequence typing (MLST), a sequence-based typing method for bacterial pathogens, is currently the best method for long-term epidemiological study and to understand the population structure of the bacteria. This investigation was carried out to study the diversity of Pasteurella multocida isolates circulating in India. Ten different sequence types (ST) identified in this study are ST 122 from cattle, goat, mithun and pig; ST 50 from pig; ST 9 from cattle and sheep; ST 229 from cattle and goat; ST 71 and ST 277 from cattle; and ST 129, ST 280, ST 281 and ST 282 from avian species. Of these, ST 277, ST 280, ST 281 and ST 282 were identified for the first time. The analysis of results provides novel epidemiological information on the circulation of multiple STs across India. The majority of STs or their variants identified in this study have already been reported from different parts of the globe. This suggests that probably transboundary spread of strains across countries and continents has occurred across evolutionary time and is still happening. The isolation of ST 122 from small ruminants and pigs suggests that these species may be included in the preventive vaccination policy for effective control of haemorrhagic septicaemia in India.

引用

页码：E286 / E292

页数：7

共 32 条

[21] Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis [J].

Moustafa, Ahmed M. ;

Bennett, Mark D. ;

Edwards, John ;

Azim, Kamran ;

Mesaik, Muhammed A. ;

Choudhary, M. Iqbal ;

Pathanasophon, Pornpen ;

Worarach, Apasara ;

Ali, Qurban ;

Abubakar, Muhammad ;

Anjum, Rehana .

RESEARCH IN VETERINARY SCIENCE, 2013, 95 (03) :986-990

[22] MLST typing of Pasteurella multocida associated with haemorrhagic septicaemia and development of a real-time PCR specific for haemorrhagic septicaemia associated isolates [J].

Petersen, Andreas ;

Bisgaard, Magne ;

Townsend, Kirsty ;

Christensen, Henrik .

VETERINARY MICROBIOLOGY, 2014, 170 (3-4) :335-341

[23]

RIRDC (Rural Industries Research and Development Corporation), 2010, MLST SCHEM P MULT

[24] Characterization of avian strains of Pasteurella multocida by restriction endonuclease and amplified fragment length polymorphism [J].

Shivachandra, SB ;

Kumar, AA ;

Gautam, R ;

Joseph, S ;

Saxena, MK ;

Chaudhuri, P ;

Srivastava, SK .

RESEARCH IN VETERINARY SCIENCE, 2006, 81 (01) :8-18

[25] Displaying the relatedness among isolates of bacterial species - the eBURST approach [J].

Spratt, BG ;

Hanage, WP ;

Li, B ;

Aanensen, DM ;

Feil, EJ .

FEMS MICROBIOLOGY LETTERS, 2004, 241 (02) :129-134

[26] Bacterial population genetics, evolution and epidemiology [J].

Spratt, BG ;

Maiden, MCJ .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1999, 354 (1384) :701-710

[27] Development of a multi-locus sequence typing scheme for avian isolates of Pasteurella multocida [J].

Subaaharan, S. ;

Blackall, L. L. ;

Blackall, P. J. .

VETERINARY MICROBIOLOGY, 2010, 141 (3-4) :354-361

[28] MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [J].

Tamura, Koichiro ;

Peterson, Daniel ;

Peterson, Nicholas ;

Stecher, Glen ;

Nei, Masatoshi ;

Kumar, Sudhir .

MOLECULAR BIOLOGY AND EVOLUTION, 2011, 28 (10) :2731-2739

[29] Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates [J].

Townsend, KM ;

Frost, AJ ;

Lee, CW ;

Papadimitriou, JM ;

Dawkins, HJS .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) :1096-1100

[30] Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system [J].

Townsend, KM ;

Boyce, JD ;

Chung, JY ;

Frost, AJ ;

Adler, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) :924-929

← 1 2 3 4 →