Three different group I introns in the nuclear large subunit ribosomal DNA of the amoeboflagellate Naegleria

We have amplified the large subunit ribosomal DNA (LSUrDNA) of the 12 described Naegleria spp, and of 34 other Naegleria lineages that might be distinct species. Two strains yielded a product that is longer than 3 kb, which is the length of the LSUrDNA of ail described Naegleria spp,Sequencing data revealed that the insert in one of these strains is a group I intron without an open reading frame (ORF), while the other strain contains two different group I introns,laf which the second intron has an ORF of 175 amino acids, in the latter ORF there is a consented Mis-Cys box, as in the homing endonucleases present in group I introns in the small subunit ribosomal DNA (SSUrDNA) of Naegleria spp. Although the group I introns in the LSUrDNA differ in sequence, they ale more related to each other than they are to the group I introns in the SSUrDNA of Naegleria spp. The three group I introns in the LSUrDNA In Naegleria are at different locations and are probably acquired by horizontal transfer, contrary to the SSUrDNA group I introns in this genus which are of ancestral origin and are transmitted vertically.