Covalent immobilization of oligonucleotides on p-aminophenyl-modified carbon screen-printed electrodes for viral DNA sensing

被引:36
作者
Ruffien, A [1 ]
Dequaire, M [1 ]
Brossier, P [1 ]
机构
[1] Fac Med & Pharm Dijon, Lab Microbiol Med & Mol, F-21000 Dijon, France
关键词
D O I
10.1039/b300439b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
DNA-sensing platforms were prepared by covalently attaching oligonucleotide capture probes onto p-aminophenyl-functionalized carbon surfaces and applied to the determination of an amplified herpes virus DNA sequence in an electrochemical hybridization assay.
引用
收藏
页码:912 / 913
页数:2
相关论文
共 17 条
[1]   METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES [J].
ALWINE, JC ;
KEMP, DJ ;
STARK, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5350-5354
[2]   Gold nanoparticle-based quantitative electrochemical detection of amplified human cytomegalovirus DNA using disposable microband electrodes [J].
Authier, L ;
Grossiord, C ;
Brossier, P ;
Limoges, B .
ANALYTICAL CHEMISTRY, 2001, 73 (18) :4450-4456
[3]   Hybridization assay at a disposable electrochemical biosensor for the attomole detection of amplified human cytomegalovirus DNA [J].
Azek, F ;
Grossiord, C ;
Joannes, M ;
Limoges, B ;
Brossier, P .
ANALYTICAL BIOCHEMISTRY, 2000, 284 (01) :107-113
[4]   Subfemtomolar determination of alkaline phosphatase at a disposable screen-printed electrode modified with a perfluorosulfonated ionomer film [J].
Bagel, O ;
Limoges, B ;
Schollhorn, B ;
Degrand, C .
ANALYTICAL CHEMISTRY, 1997, 69 (22) :4688-4694
[5]   Modification of carbon fiber surfaces by electrochemical reduction of aryl diazonium salts: Application to carbon epoxy composites [J].
Delamar, M ;
Desarmot, G ;
Fagebaume, O ;
Hitmi, R ;
Pinson, J ;
Saveant, JM .
CARBON, 1997, 35 (06) :801-807
[6]   Robust and efficient synthetic method for forming DNA microarrays [J].
Dolan, PL ;
Wu, Y ;
Ista, LK ;
Metzenberg, RL ;
Nelson, MA ;
Lopez, GP .
NUCLEIC ACIDS RESEARCH, 2001, 29 (21) :107-107
[7]   PROTEIN ADSORPTION AT GLASSY-CARBON ELECTRODES - THE EFFECT OF COVALENTLY BOUND SURFACE GROUPS [J].
DOWNARD, AJ ;
RODDICK, AD .
ELECTROANALYSIS, 1995, 7 (04) :376-378
[8]   Development of a molecular diagnosis assay based on electrohybridization at plastic electrodes and subsequent PCR [J].
Hassmann, J ;
Misch, A ;
Schülein, J ;
Krause, J ;
Grassl, B ;
Müller, P ;
Bertling, WM .
BIOSENSORS & BIOELECTRONICS, 2001, 16 (9-12) :857-863
[9]   Label-free determination of picogram quantities of DNA by stripping voltammetry with solid copper amalgam or mercury electrodes in the presence of copper [J].
Jelen, F ;
Yosypchuk, B ;
Kourilová, A ;
Novotny, L ;
Palecek, E .
ANALYTICAL CHEMISTRY, 2002, 74 (18) :4788-4793
[10]   Disposable DNA electrochemical sensor for hybridization detection [J].
Marrazza, G ;
Chianella, I ;
Mascini, M .
BIOSENSORS & BIOELECTRONICS, 1999, 14 (01) :43-51