Evaluation of the biocompatibility of acellular porcine dermis

被引：22

作者：

Tang, Li-Ling ^{[1
]}

Liu, Huan ^{[1
]}

Wang, Yuan-Liang ^{[1
]}

Man, Cheng-Yu ^{[1
]}

Su, Ai-Hua ^{[1
]}

机构：

[1] Chongqing Univ, Coll Bioengn, Key Lab Biomech & Tissue Engn, Minist Educ, Chongqing 400044, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2007年 / 57卷 / 02期

关键词：

porcine dermis; biocompatibility; morphology; adhesive force; integrin; CULTURED EPIDERMAL ALLOGRAFTS; CELL-ADHESION; SKIN; TISSUE; ANGIOGENESIS; REPLACEMENT; GRAFT;

D O I：

10.1016/j.colsurfb.2007.02.009

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

The biocompatibility of an acellular porcine dermis was evaluated by in vitro methods. Endothelia cells (ECV-304) and fibroblasts (NIH3T3) were seeded on the dermis and cultured for I week to assess the cell viability in the skin grafts. Results by morphological assessment and methylthiazolyl tetrazolium assay (MTT assay) indicated good biocompatibility of acellular porcine dermis, which allowed adhesion and proliferation of examined cell types. Flow chamber technique was used to evaluate the adhesive force between cells and biomaterials. There was no significant difference in cells retention ratio between control cells and experimental cells after sheared 24 h. The determination of integrin alpha 5 expression proved that the acellular porcine dermis did not influence the expression of integrin a5 in cells. The results suggested that the dermal equivalent made from pigskin is a promising material for burn treatment (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：215 / 218

页数：4

共 18 条

[1]

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