Hv-WRKY38:: a new transcription factor involved in cold- and drought-response in barley

被引:255
作者
Marè, C
Mazzucotelli, E
Crosatti, C
Francia, E
Stanca, AM
Cattivelli, L
机构
[1] Ist Sperimentale Cerealicoltura, I-29017 Fiorenzuola Darda, PC, Italy
[2] Ist Sperimentale Cerealicoltura, I-71100 Foggia, Italy
[3] Univ Ferrara, Dipartimento Biol, Sez Biol Evolut, I-44100 Ferrara, Italy
关键词
barley; cold; drought; freezing; transcription factor; WRKY protein;
D O I
10.1007/s11103-004-0906-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
WRKY proteins constitute a large family of plant specific transcription factors implicated in many different processes. Here we describe Hv-WRKY38, a barley gene coding for a WRKY protein, whose expression is involved in cold and drought stress response. Hv-WRKY38 was early and transiently expressed during exposure to low non-freezing temperature, in ABA-independent manner. Furthermore, it showed a continuous induction during dehydration and freezing treatments. A WRKY38:YFP fusion protein was found to localise into the nucleus upon introduction into epidermal onion cells. Bacterially expressed Hv-WRKY38 was able to bind in vitro to the W-box element (T)TGAC(C/T) also recognisable by other WRKY proteins. Hv-WRKY38 genomic DNA was sequenced and mapped onto the centromeric region of the barley chromosome 6H. Arabidopsis and rice sequences homologous to Hv-WRKY38 were also identified. Our results indicate that Hv-WRKY38 transcription factor may play a regulatory role in abiotic stress response.
引用
收藏
页码:399 / 416
页数:18
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