First histopathological and immunophenotypic analysis of early dynamic events in a patient with segmental vitiligo associated with halo nevi

被引:71
作者
van Geel, Nanja A. C. [1 ]
Mollet, Ilse G. [1 ]
De Schepper, Sofie [1 ]
Tjin, Esther P. M. [2 ,3 ]
Vermaelen, Karim [4 ]
Clark, Rachael A. [5 ,6 ]
Kupper, Thomas S. [5 ,6 ]
Luiten, Rosalie M. [2 ,3 ]
Lambert, Jo [1 ]
机构
[1] Ghent Univ Hosp, Dept Dermatol, B-9000 Ghent, Belgium
[2] Univ Amsterdam, Acad Med Ctr, Dept Dermatol, NL-1105 AZ Amsterdam, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Netherlands Inst Pigment Disorders, NL-1105 AZ Amsterdam, Netherlands
[4] Ghent Univ Hosp, Dept Pulm Med, Lab Immunoregulat & Mucosal Immunol, B-9000 Ghent, Belgium
[5] Brigham & Womens Hosp, Dept Dermatol, Boston, MA 02115 USA
[6] Brigham & Womens Hosp, Harvard Skin Dis Res Ctr, Boston, MA 02115 USA
关键词
vitiligo; segmental vitiligo; etiopathogenesis; histopathology; melanocyte; halo nevi; REGULATORY T-CELLS; IMMUNE-RESPONSE; HUMAN SKIN; LESIONS; MELANOCYTES; TH17; PATHOGENESIS; INFLAMMATION; DESTRUCTION; EXPRESSION;
D O I
10.1111/j.1755-148X.2010.00703.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
P>Segmental vitiligo is often ascribed to the neurogenic theory of melanocyte destruction, although data about the initial etiopathological events are scarce. Clinical, histopathological and T-cell phenotypic analyses were performed during the early onset of a segmental vitiligo lesion in a patient with associated halo nevi. Histopathological analysis revealed a lymphocytic infiltrate, mainly composed of CD8+ T-cells and some CD4+ T-cells around the dermo-epidermal junction. Flow cytometry analysis of resident T-cells revealed a clear enrichment of pro-inflammatory IFN-gamma producing CD8+ T-cells in lesional skin compared to the non-lesional skin. Using human leukocyte antigen-peptide tetramers (MART-1, tyrosinase, gp100), increased numbers of T cells, recognizing melanocyte antigens were found in segmental vitiligo lesional skin, as compared with the non-lesional skin and the blood. Our findings indicate that a CD8+ melanocyte specific T cell-mediated immune response, as observed in generalized vitiligo, also plays a role in segmental vitiligo with associated halo nevi.
引用
收藏
页码:375 / 384
页数:10
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