Dielectrophoretic manipulation of rod-shaped viral particles

被引:192
作者
Morgan, H [1 ]
Green, NG [1 ]
机构
[1] Univ Glasgow, Bioelect Res Ctr, Glasgow G12 8QQ, Lanark, Scotland
基金
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词
dielectrophoresis; viral particles; tobacco mosaic virus; micro-electrodes; submicron particle collection;
D O I
10.1016/S0304-3886(97)00159-9
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
The dielectrophoretic manipulation of a rod-shaped virus, tobacco mosaic virus (TMV) by means of non-uniform AC electric fields is described. An expression is derived for the dielectrophoretic force on a homogeneous dielectric cylinder suspended in a liquid medium which shows that the dielectrophoretic force varies over a wide range of frequencies and medium conductivities. Measurements of the dielectrophoretic behaviour of TMV particles in microelectrode arrays have been made as a function of frequency and applied field strength. The results are shown to be in quantitative agreement with the derived expression for the dielectrophoretic force. By measuring the threshold field strength for the onset of dielectrophoresis, the force on a single TMV particle has been estimated. The results point to the potential use of dielectrophoresis for the collection and manipulation of sub-micron particles using microelectrode arrays. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:279 / 293
页数:15
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