DNA replication and transcription direct a DNA strand bias in the process of targeted gene repair in mammalian cells

被引:42
作者
Brachman, EE [1 ]
Kmiec, EB [1 ]
机构
[1] Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA
关键词
DNA repair; SV40; COS cells;
D O I
10.1242/jcs.01250
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The repair of point mutations can be directed by modified single-stranded DNA oligonucleotides and regulated by cellular activities including homologous recombination, mismatch repair and transcription. Now, we report that DNA replication modulates the gene repair process by influencing the frequency with which either DNA strand is corrected. An SV40-virus-based system was used to investigate the role of DNA synthesis on gene repair in COS-1 cells. We confirm that transcription exerts a strand bias on the gene repair process even when correction takes place on actively replicating templates. We were able to distinguish between the influences of transcription and replication on strand bias by changing the orientation of a gene encoding enhanced green fluorescent protein relative to the origin of replication, and confirmed the previously observed bias towards the untranscribed strand. We report that DNA replication can increase the level of untranscribed strand preference only if that strand also serves as the lagging strand in DNA synthesis. Furthermore, the effect of replication on gene repair frequency and strand bias appears to be independent of certain mismatched base pairs and oligonucleotide length.
引用
收藏
页码:3867 / 3874
页数:8
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