Electroporation as a tool to study in vivo spinal cord regeneration

被引:40
作者
Echeverri, K [1 ]
Tanaka, EM [1 ]
机构
[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
关键词
spinal cord; electroporation; regeneration;
D O I
10.1002/dvdy.10238
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Tailed amphibians such as axolotls and newts have the unique ability to fully regenerate a functional spinal cord throughout life. Where the cells come from and how they form the new structure is still poorly understood. Here, we describe the development of a technique that allows the visualization of cells in the living animal during spinal cord regeneration. A microelectrode needle is inserted into the lumen of the spinal cord and short rapid pulses are applied to transfer the plasmids encoding the green or red fluorescent proteins into ependymal cells close to the plane of amputation. The use of small, transparent axolotls permits imaging with epifluorescence and differential interference contrast microscopy to track the transfected cells as they contribute to the spinal cord. This technique promises to be useful in understanding how neural progenitors are recruited to the regenerating spinal cord and opens up the possibility of testing gene function during this process. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:418 / 425
页数:8
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