Neurotrophic properties of olfactory ensheathing glia

被引：221

作者：

Lipson, AC

Widenfalk, J

Lindqvist, E

Ebendal, T

Olson, L ^{[1
]}

机构：

[1] Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden

[2] Univ Washington, Dept Neurol Surg, Seattle, WA 98105 USA

[3] Uppsala Univ, Dev Biol Unit, Dept Neurosci, S-75123 Uppsala, Sweden

来源：

EXPERIMENTAL NEUROLOGY | 2003年 / 180卷 / 02期

关键词：

olfactory ensheathing glia; axonal regeneration; olfactory nerve; neurotrophic factor; osteogenic protein-1; artemin; GDNF; neurotrophin; S-100; in situ hybridization;

D O I：

10.1016/S0014-4886(02)00058-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Olfactory ensheathing cells (OEC) constitute a specialized population of glia that accompany primary olfactory axons and have been reported to facilitate axonal regeneration after spinal cord injury in vivo. In the present report we describe OEC neurotrophic factor expression and neurotrophic properties of OECs in vitro. Investigation of the rat olfactory system during development and adulthood by radioactive in situ hybridization revealed positive labeling in the olfactory nerve layer for the neurotrophic molecules S-100beta, CNTF, BMP-7/OP-1, and artemin, as well as for the neurotrophic factor receptors RET and TrkC. Ribonuclease protection assay of cultured. OEC revealed expression of NGF, BDNF, GDNF, and CNTF mRNA, while NT3 and NT4 mRNA were not detectable. In vitro bioassays of neurotrophic activity involved coculturing of adult OEC with embryonic chick ganglia and demonstrated increased neurite outgrowth from sympathetic, ciliary, and Remak's ganglia. However, when culturing the ganglia with OEC-conditioned medium, neurite outgrowth was not stimulated to any detectable extent. Our results suggest that the neurotrophic properties of OEC may involve secretion of neurotrophic molecules but that cellular interactions are crucial. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：167 / 171

页数：5

共 44 条

[1] Artemin, a novel member of the GDNF ligand family, supports peripheral and central neurons and signals through the GFRα3-RET receptor complex [J].