Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not

被引：41

作者：

Wiggerich, HG ^{[1
]}

Klauke, B ^{[1
]}

Koplan, R ^{[1
]}

Priefer, UB ^{[1
]}

Puhler, A ^{[1
]}

机构：

[1] UNIV BIELEFELD,FAK BIOL,LEHRSTUHL GENET,D-33501 BIELEFELD,GERMANY

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 22期

关键词：

D O I：

10.1128/jb.179.22.7103-7110.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequence of a 3.6-kb HindIII-SmaI DNA fragment of Xanthomonas campestris pv. campestris revealed four open reading frames which, based on sequence homologies, were designated tonB, exbB, exbD1, and exbD2. Analysis of translational fusions to alkaline phosphatase and beta-galactosidase confirmed that the TonB, ExbB, ExbD1, and ExbD2 proteins are anchored in the cytoplasmic membrane. The TonB protein of X. campestris pv. campestris lacks the conserved (Glu-Pro)(n) and (Lys-Pro)(m) repeats but harbors a 13-fold repeat of proline residues. By mutational analysis, the tonB, exbB, and exbD1 genes were shown to be essential for ferric iron import in X. campestris pv. campestris. In contrast, the exbD2 gene is not involved in the uptake of ferric iron.

引用

页码：7103 / 7110

页数：8

共 61 条

[1] CHARACTERIZATION OF A RHIZOBIUM-MELILOTI FIXATION GENE (FIXF) LOCATED NEAR THE COMMON NODULATION REGION [J].

AGUILAR, OM ;

KAPP, D ;

PUHLER, A .

JOURNAL OF BACTERIOLOGY, 1985, 164 (01) :245-254

[2] CHARACTERIZATION OF THE EXBBD OPERON OF ESCHERICHIA-COLI AND THE ROLE OF EXBB AND EXBD IN TONB FUNCTION AND STABILITY [J].

AHMER, BMM ;

THOMAS, MG ;

LARSEN, RA ;

POSTLE, K .

JOURNAL OF BACTERIOLOGY, 1995, 177 (16) :4742-4747

[3] A FAMILY OF HIGH-COPY-NUMBER PLASMID VECTORS WITH SINGLE END-LABEL SITES FOR RAPID NUCLEOTIDE SEQUENCING [J].

ARNOLD, W ;

PUHLER, A .

GENE, 1988, 70 (01) :171-179

[4]

BACHMAN K, 1976, P NATL ACAD SCI USA, V73, P4174

[5] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197

[6] NEW GENTAMICIN-RESISTANCE AND LACZ PROMOTER-PROBE CASSETTES SUITABLE FOR INSERTION MUTAGENESIS AND GENERATION OF TRANSCRIPTIONAL FUSIONS [J].

BECKER, A ;

SCHMIDT, M ;

JAGER, W ;

PUHLER, A .

GENE, 1995, 162 (01) :37-39

[7] LOW-MOLECULAR-WEIGHT SUCCINOGLYCAN IS PREDOMINANTLY PRODUCED BY RHIZOBIUM-MELILOTI STRAINS CARRYING A MUTATED EXOP PROTEIN CHARACTERIZED BY A PERIPLASMIC N-TERMINAL DOMAIN AND A MISSING C-TERMINAL DOMAIN [J].

BECKER, A ;

NIEHAUS, K ;

PUHLER, A .

MOLECULAR MICROBIOLOGY, 1995, 16 (02) :191-203

[8] The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: Genetic organization and properties of the encoded gene products [J].

Becker, A ;

Ruberg, S ;

Kuster, H ;

Roxlau, AA ;

Keller, M ;

Ivashina, T ;

Cheng, HP ;

Walker, GC ;

Puhler, A .

JOURNAL OF BACTERIOLOGY, 1997, 179 (04) :1375-1384

[9] IDENTIFICATION AND CHARACTERIZATION OF THE EXBB, EXBD AND TONB GENES OF PSEUDOMONAS-PUTIDA WCS358 - THEIR INVOLVEMENT IN FERRIC-PSEUDOBACTIN TRANSPORT [J].

BITTER, W ;

TOMMASSEN, J ;

WEISBEEK, PJ .

MOLECULAR MICROBIOLOGY, 1993, 7 (01) :117-130

[10] THE PROTON MOTIVE FORCE DRIVES THE OUTER-MEMBRANE TRANSPORT OF COBALAMIN IN ESCHERICHIA-COLI [J].

BRADBEER, C .

JOURNAL OF BACTERIOLOGY, 1993, 175 (10) :3146-3150

← 1 2 3 4 5 6 7 →