First evidence of palytoxin analogues from an Ostreopsis mascarenensis (Dinophyceae) benthic bloom in Southwestern Indian Ocean

被引:136
作者
Lenoir, S
Ten-Hage, L [1 ]
Turquet, J
Quod, JP
Bernard, C
Hennion, MC
机构
[1] Univ Toulouse 3, CNRS, FRE 2630, Lab Ecol Hydrosyst, F-31062 Toulouse, France
[2] Ecole Super Phys & Chim Ind Ville Paris, CNRS, UMR 7121, Lab Environm & Chim Analyt, F-75231 Paris, France
[3] Agcy Rech & Valorisat Marines, F-97490 Ste Clotilde, France
[4] Museum Natl Hist Nat, Unite Interact Tox Ecosyst, F-75231 Paris, France
关键词
benthic dinoflagellates; clupeotoxism; Indian Ocean; mascarenotoxins; mass spectrometry; Ostreopsis mascarenensis; palytoxin;
D O I
10.1111/j.1529-8817.2004.04016.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Benthic dinoflagellates of the genus Ostreopsis Schmidt are common in tropical and subtropical water, and some species produce toxins potentially involved in human intoxication events. A benthic bloom of Ostreopsis mascarenensis Quod was observed near Rodrigues Island during a survey of benthic dinoflagellates in the southwestern Indian Ocean. The morphology of O. mascarenensis was studied by LM and SEM. Preliminary screening of a crude extract of an O. mascarenensis bloom revealed neurotoxicity in mice similar to that induced by palytoxin. After partition of the crude extract, the highest toxicity was retained in the butanol-soluble fraction, which retained hemolytic activity suggestive of palytoxin analogues. Two new toxins, mascarenotoxin-A and -B, were resolved from this fraction by HPLC coupled to a diode array detector. The closed mass spectrum profile and fragmentation pattern obtained by advanced nano-electrospray ionization quadrupole time-of-flight mass spectrometry between purified toxins and a reference palytoxin confirmed the mascarenotoxins as palytoxin analogues. These results were confirmed by tandem mass spectrometry with the identification of specific fragment ion m/z 327. An on-line liquid chromatography protocol coupled to tandem mass spectrometry was developed for detection of these palytoxin analogues. The present study describes the first purification, chemical, and toxicological characterization of new palytoxin analogues isolated from a benthic bloom of O. mascarenensis. These results suggest that O. mascarenensis, which is largely distributed in the southwestern Indian Ocean, could be a source of palytoxin poisoning in this tropical area.
引用
收藏
页码:1042 / 1051
页数:10
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