Two new monogalactosyl diacylglycerols from brown alga Sargassum thunbergii

被引：46

作者：

Kim, Young Hwan

Kim, Eun-Hee

Lee, Chulhyun

Kim, Mi-Hee

Rho, Jung-Rae

机构：

[1] Kunsan Natl Univ, Dept Oceanog, Kunsan 573701, South Korea

[2] Korea Basic Sci Inst, Magnet Resonance Team, Taejon 305333, South Korea

[3] Korea Basic Sci Inst, Proteom Team, Taejon 305333, South Korea

来源：

LIPIDS | 2007年 / 42卷 / 04期

关键词：

Sargassum thunbergii; monogalactosyl diacylglycerols (MGDGs); collision-induced dissociation (CID); fast atom bombardment (FAB); heteronuclear multiple bond coherence (HMBC); chromatography; regiospecificity; (omega-3) polyunsaturated fatty acid;

D O I：

10.1007/s11745-007-3035-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Two new monogalactosyl diacylglycerols (MGDGs) along with two known glycolipids were isolated from the moderate polar fraction of the methanolic extract of the brown alga Sargassum thunbergii by using reversed silica flash chromatography. Two new MGDGs were identified as (2S)-1-O-(5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl) -2-O-(9Z, 12Z, 15Z-octadecatrienoyl)-3-O-beta-D-galactopyranosyl-sn-glycerol (1) and (2S)-1-O-(9Z,12Z,15Z-octadecatrienoyl)-2-O-(6Z,9Z,12Z,15Z-octadecatetraenoyl)-3-O -beta-D-galactopyranosyl-sn-glycerol (2) by FAB tandem mass spectrometry, NMR techniques, and specific enzymecatalyzed hydrolysis of the sn-1 fatty acyl linkage. The regiochemical attachment of the acyl chains in the glycerol moiety was established by 2D NMR correlations and confirmed by enzymatic hydrolysis.

引用

页码：395 / 399

页数：5

共 11 条

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ITO H, 1976, CHEM PHARM BULL, V24, P1114

[2]

Diacylglycerylgalactosides from Arisaema amurense [J].