Enhancements and modifications of primer design program Primer3

被引：2085

作者：

Koressaar, Triinu ^{[1
]}

Remm, Maido ^{[1
]}

机构：

[1] Univ Tartu, Inst Mol & Cell Biol, Dept Bioinformat, EE-51010 Tartu, Estonia

来源：

BIOINFORMATICS | 2007年 / 23卷 / 10期

关键词：

D O I：

10.1093/bioinformatics/btm091

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of annealing temperature is a critical step in PCR design. This parameter is typically derived from the melting temperature of the PCR primers, so for successful PCR work it is important to determine the melting temperature of primer accurately. We introduced several enhancements in the widely used primer design program Primer3. The improvements include a formula for calculating melting temperature and a salt correction formula. Also, the new version can take into account the effects of clivalent cations, which are included in most PCR buffers. Another modification enables using lowercase masked template sequences for primer design.

引用

页码：1289 / 1291

页数：3

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