Transcriptome profiling of Lotus japonicus roots during arbuscular mycorrhiza development and comparison with that of nodulation

被引:52
作者
Deguchi, Yuichi
Banba, Mari
Shimoda, Yoshikazu
Chechetka, Svetlana A.
Suzuri, Ryota
Okusako, Yasuhiro
Ooki, Yasuhiro
Toyokura, Koichi
Suzuki, Akihiro
Uchiumi, Toshiki
Higashi, Shiro
Abe, Mikiko
Kouchi, Hiroshi
Izui, Katsura
Hata, Shingo [1 ]
机构
[1] Kyoto Univ, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068502, Japan
[2] Kagoshima Univ, Grad Sch Sci & Technol, Kagoshima 8900065, Japan
[3] Kagoshima Univ, Dept Chem & Biosci, Kagoshima 8900065, Japan
[4] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
关键词
cysteine proteinase; defense response; phenylalanine ammonia-lyase; symbiosis;
D O I
10.1093/dnares/dsm014
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To better understand the molecular responses of plants to arbuscular mycorrhizal (AM) fungi, we analyzed the differential gene expression patterns of Lotus Japonicus, a model legume, with the aid of a large-scale cDNA macro-array. Experiments were carried out considering the effects of contaminating microorganisms in the soil inoculants. When the colonization by AM fungi, i.e. Glomus mosseae and Gigaspora margarita, was well established, four cysteine protease genes were induced. In situ hybridization revealed that these cysteine protease genes were specifically expressed in arbuscule-containing inner cortical cells of AM roots. On the other hand, phenylpropanoid biosynthesis-related genes for phenylalanine ammonia-lyase (PAL), chalcone synthase, etc. were repressed in the later stage, although they were moderately up-regulated on the initial association with the AM fungus. Real-time RT-PCR experiments supported the array experiments. To further confirm the characteristic expression, a PAL promoter was fused with a reporter gene and introduced into L. japonicus, and then the transformants were grown with a commercial inoculum of G. mosseae. The reporter activity was augmented throughout the roots due to the presence of contaminating microorganisms in the inoculum. Interestingly, G. mosseae only colonized where the reporter activity was low. Comparison of the transcriptome profiles of AM roots and nitrogen-fixing root nodules formed with Mesorhizobium loti indicated that the PAL genes and other phenylpropanoid biosynthesis-related genes were similarly repressed in the two organs.
引用
收藏
页码:117 / 133
页数:17
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