A new monoclonal antibody detecting ochratoxin A at the picogram level

被引：21

作者：

GyongyosiHorvath, A

BarnaVetro, I

Solti, L

机构：

[1] Agricultural Biotechnology Center, Gödöllö

[2] Agricultural Biotechnology Center, H-2101 Gödöllö

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 22卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1996.tb01119.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A monoclonal antibody against ochratoxin A was produced after immunization of Balb/c mice with ochratoxin A-BSA. This antibody was of the IgG1 heavy chain subclass with a kappa type light chain. The 50% inhibition value was 0.45 ng ml(-1) in a direct competitive ELISA and the detection limit was 42 pg ml(-1). This antibody is very specific, cross-reacting only with ochratoxin B (9.3%).

引用

页码：103 / 105

页数：3

共 14 条

[1] A MONOCLONAL-ANTIBODY TO OCHRATOXIN-A [J].

CANDLISH, AAG ;

STIMSON, WH ;

SMITH, JE .

LETTERS IN APPLIED MICROBIOLOGY, 1986, 3 (01) :9-11

[2]

CHIBA J, 1988, FOOD ADDIT CONTAM, V5, P629, DOI 10.1080/02652038809373727

[3] PRODUCTION OF ANTIBODY AGAINST OCHRATOXIN-A [J].

CHU, FS ;

CHANG, FCC ;

HINSDILL, RD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1976, 31 (06) :831-835

[4] A MONOCLONAL-ANTIBODY TO THE TRICHOTHECENE T-2 TOXIN - SCREENING FOR THE ANTIBODY BY A DIRECT ENZYME-IMMUNOASSAY [J].

HACK, R ;

MARTLBAUER, E ;

TERPLAN, G .

JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1987, 34 (07) :538-544

[5] A SENSITIVE ENZYME-LINKED IMMUNOSORBENT-ASSAY OF OCHRATOXIN-A BASED ON MONOCLONAL-ANTIBODIES [J].

KAWAMURA, O ;

SATO, S ;

KAJII, H ;

NAGAYAMA, S ;

OHTANI, K ;

CHIBA, J ;

UENO, Y .

TOXICON, 1989, 27 (08) :887-897

[6]

KOEHLER G, 1975, NATURE, V256, P495

[7]

MARTLBAUER E, 1991, ARCH LEBENSMITTELHYG, V42, P3

[8]

Martlbauer E., 1988, ARCH LEBENSMITTELHYG, V39, P133

[9]

MORGAN MRA, 1983, J ASSOC OFF ANA CHEM, V66, P1481

[10]

Oi V.T., 1980, SELECTED METHODS CEL, P351

← 1 2 →