Nitric oxide donors release extracellular traps from human neutrophils by augmenting free radical generation

被引：128

作者：

Patel, Satyananda ^{[1
]}

Kumar, Sachin ^{[1
]}

Jyoti, Anupam ^{[1
]}

Srinag, Bangalore Suresh ^{[4
]}

Keshari, Ravi Shankar ^{[1
]}

Saluja, Rohit ^{[1
]}

Verma, Anupam ^{[3
]}

Mitra, Kalyan ^{[2
]}

Barthwal, Manoj Kumar ^{[1
]}

Krishnamurthy, Hanumanthappa ^{[4
]}

Bajpai, Virendra K. ^{[2
]}

Dikshit, Madhu ^{[1
]}

机构：

[1] Cent Drug Res Inst, Cardiovasc Pharmacol Unit, Council Sci & Ind Res, Lucknow 226001, Uttar Pradesh, India

[2] Cent Drug Res Inst, Electron Microscopy Unit, Council Sci & Ind Res, Lucknow 226001, Uttar Pradesh, India

[3] Sanjay Gandhi Postgrad Inst Med Sci, Lucknow, Uttar Pradesh, India

[4] Natl Ctr Biol Sci, Cent Image Flow Facil, Bangalore, Karnataka, India

来源：

NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2010年 / 22卷 / 03期

关键词：

Neutrophils extracellular traps; Nitric oxide; NADPH-oxidase; Free radicals; Myeloperoxidase; FLOW-CYTOMETRIC ANALYSIS; TOLL-LIKE RECEPTOR-4; OXIDATIVE STRESS; SYNTHASE; INHIBITION; CELLS; MYELOPEROXIDASE; STREPTOCOCCUS; LOCALIZATION; EXPRESSION;

D O I：

10.1016/j.niox.2010.01.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High availability of NO, oxidative stress and neutrophil extracellular trap (NETs) contents are often noticed at the site of inflammation/infection. Studies from this lab and others have reported NO mediated free radical generation from neutrophils: role of NO in NETs formation however remains undefined so far. The present study was therefore undertaken to explore the effect of No donors on NET release from human neutrophils (PMNs), using confocal/scanning microscopy, measuring the extracellular DNA content and NET-bound elastase activity. Addition of NO donors (SNAP and SNP) to adhered PMNs led to a time and concentration dependent NETs release, which was blocked by N-acetyl cysteine, suggesting involvement of free radicals in NETs formation. Free radical formation by NO donors was assessed by using DCF-DA, DMPO-nitrone antibody and by p47 phox migration to the neutrophils membrane. NO mediated formation of free radicals and NETs was significantly reduced by the pretreatment of neutrophils with diphenyleneiodonium (DPI), a NADPH-oxidase inhibitor and 4-aminobenzoic acid hydrazide (ABAH), a myeloperoxidase inhibitor, suggesting role of enzymatic free radical generation by NO donors. We thus demonstrate that NO by augmenting free radical formation in human neutrophils mediates NETs release. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：226 / 234

页数：9

共 53 条

[1] Nitric oxide is a physiological substrate for mammalian peroxidases [J].

Abu-Soud, HM ;

Hazen, SL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (48) :37524-37532

[2] Inhibition of the spontaneous apoptosis of neutrophil granulocytes by the intracellular parasite Leishmania major [J].

Aga, E ;

Katschinski, DM ;

van Zandbergen, G ;

Laufs, H ;

Hansen, B ;

Müller, K ;

Solbach, W ;

Laskay, T .

JOURNAL OF IMMUNOLOGY, 2002, 169 (02) :898-905

[3] The neutrophil NADPH oxidase [J].

Babior, BM ;

Lambeth, JD ;

Nauseef, W .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2002, 397 (02) :342-344

[4] Cytokine-associated neutrophil extracellular traps and antinuclear antibodies in Plasmodium falciparum infected children under six years of age [J].

Baker, Virginia S. ;

Imade, Godwin E. ;

Molta, Norman B. ;

Tawde, Pallavi ;

Pam, Sunday D. ;

Obadofin, Michael O. ;

Sagay, Soloman A. ;

Egah, Daniel Z. ;

Iya, Daniel ;

Afolabi, Bangmboye B. ;

Baker, Murray ;

Ford, Karen ;

Ford, Robert ;

Roux, Kenneth H. ;

Keller, Thomas Cs .

MALARIA JOURNAL, 2008, 7 (1)

[5] Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation [J].

Beck, Paul L. ;

Li, Yan ;

Wong, J. ;

Chen, Chang-Wen ;

Keenan, Catherine M. ;

Sharkey, Keith A. ;

McCafferty, Donna-Marie .

GASTROENTEROLOGY, 2007, 132 (05) :1778-1790

[6] An endonuclease allows Streptococcus pneumoniae to escape from neutrophil extracellular traps [J].

Beiter, K ;

Wartha, F ;

Albiger, B ;

Normark, S ;

Zychlinsky, A ;

Henriques-Normark, B .

CURRENT BIOLOGY, 2006, 16 (04) :401-407

[7] Inhibition of leukocyte rolling by nitric oxide during sepsis leads to reduced migration of active microbicidal neutrophils [J].

Benjamim, CF ;

Silva, JS ;

Fortes, ZB ;

Oliveira, MA ;

Ferreira, SH ;

Cunha, FQ .

INFECTION AND IMMUNITY, 2002, 70 (07) :3602-3610

[8] Neutrophil extracellular traps kill bacteria [J].

Brinkmann, V ;

Reichard, U ;

Goosmann, C ;

Fauler, B ;

Uhlemann, Y ;

Weiss, DS ;

Weinrauch, Y ;

Zychlinsky, A .

SCIENCE, 2004, 303 (5663) :1532-1535

[9] DNase expression allows the pathogen group A streptococcus to escape killing in neutrophil extracellular traps [J].

Buchanan, JT ;

Simpson, AJ ;

Aziz, RK ;

Liu, GY ;

Kristian, SA ;

Kotb, M ;

Feramisco, J ;

Nizet, V .

CURRENT BIOLOGY, 2006, 16 (04) :396-400

[10] Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood [J].

Clark, Stephen R. ;

Ma, Adrienne C. ;

Tavener, Samantha A. ;

McDonald, Braedon ;

Goodarzi, Zahra ;

Kelly, Margaret M. ;

Patel, Kamala D. ;

Chakrabarti, Subhadeep ;

McAvoy, Erin ;

Sinclair, Gary D. ;

Keys, Elizabeth M. ;

Allen-Vercoe, Emma ;

DeVinney, Rebekah ;

Doig, Christopher J. ;

Green, Francis H. Y. ;

Kubes, Paul .

NATURE MEDICINE, 2007, 13 (04) :463-469

← 1 2 3 4 5 6 →