Pulsed plasma deposition of allylamine on polysiloxane: a stable surface for neuronal cell adhesion

被引:99
作者
Harsch, A
Calderon, J
Timmons, RB
Gross, GW
机构
[1] Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA
[2] Univ N Texas, Ctr Network Neurosci, Denton, TX 76203 USA
[3] Univ Cambridge, Dept Physiol, Cambridge CB2 3EG, England
[4] Univ Texas, Dept Chem & Biochem, Arlington, TX 76019 USA
关键词
cell culture; spinal tissue; plasma-modified surfaces;
D O I
10.1016/S0165-0270(00)00196-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Allylamine was pulse-plasma polymerized onto a hydrophobic polysiloxane substrate to create cell adhesion surfaces for cell culture that would not require pretreatment with polylysine, could be sterilized via autoclaving, and could be re-used for several culture cycles. We investigated two different plasma deposition protocols at 200 W RF power: (1) a duty cycle of 3 ms on and 5 ms off; and (2) a cycle of 3 ms on and 45 ms off. Control surfaces were unmodified polysiloxane, activated polysiloxane via flaming, and flamed polysiloxane further modified with poly-D-lysine (PDL). The different surfaces were characterized with XPS analysis, water contact angle, and cell adhesion and growth using dissociated murine embryonic spinal tissue. We found that both the amine content of the 3/45 duty cycle surface and the wettability was higher than that of the 3/5 surface. Also, spinal cord cells were better dispersed 24 h after seeding on the 3/45 surface, suggesting a difference in early adhesion dynamics. However, the networks on the two types of modified surfaces revealed no obvious morphological differences after 2 weeks in vitro. The stability of allylamine-decorated surfaces after autoclaving was high with only minor changes in wettability and nitrogen content. Cell growth on such surfaces after autoclaving was comparable to that found on flamed polysiloxane, freshly modified with PDL. Allylamine surfaces were still usable as cell growth substrates after three autoclaving cycles, 4 weeks under warm culture medium, and simple cleaning procedures, indicating the achievement of a long-lasting modification that did not require the repeated use of PDL before each seeding. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:135 / 144
页数:10
相关论文
共 45 条
  • [1] [Anonymous], ENABLING TECHNOLOGIE
  • [2] FABRICATION OF SURFACES RESISTANT TO PROTEIN ADSORPTION AND APPLICATION TO 2-DIMENSIONAL PROTEIN PATTERNING
    BHATIA, SK
    TEIXEIRA, JL
    ANDERSON, M
    SHRIVERLAKE, LC
    CALVERT, JM
    GEORGER, JH
    HICKMAN, JJ
    DULCEY, CS
    SCHOEN, PE
    LIGLER, FS
    [J]. ANALYTICAL BIOCHEMISTRY, 1993, 208 (01) : 197 - 205
  • [3] Surface molecular tailoring via pulsed plasma-generated acryloyl chloride polymers: Synthesis and reactivity
    Calderon, JG
    Timmons, RB
    [J]. MACROMOLECULES, 1998, 31 (10) : 3216 - 3224
  • [4] Calderon JG, 1998, J BIOMED MATER RES, V42, P597, DOI 10.1002/(SICI)1097-4636(19981215)42:4<597::AID-JBM16>3.0.CO
  • [5] 2-R
  • [6] PHOTOIMMOBILIZATION OF A BIOACTIVE LAMININ FRAGMENT AND PATTERN-GUIDED SELECTIVE NEURONAL CELL ATTACHMENT
    CLEMENCE, JF
    RANIERI, JP
    AEBISCHER, P
    SIGRIST, H
    [J]. BIOCONJUGATE CHEMISTRY, 1995, 6 (04) : 411 - 417
  • [7] DROGE MH, 1986, J NEUROSCI, V6, P1583
  • [8] NEURONS FROM FETAL RAT-BRAIN IN A NEW CELL-CULTURE SYSTEM - MULTIDISCIPLINARY ANALYSIS
    GODFREY, EW
    NELSON, PG
    SCHRIER, BK
    BREUER, AC
    RANSOM, BR
    [J]. BRAIN RESEARCH, 1975, 90 (01) : 1 - 21
  • [9] ALCOHOLIC BOUIN FIXATION OF INSECT NERVOUS SYSTEMS FOR BODIAN SILVER STAINING .3. A SHORTENED, SINGLE IMPREGNATION METHOD
    GREGORY, GE
    [J]. STAIN TECHNOLOGY, 1980, 55 (03): : 161 - 165
  • [10] GROWTH OF HUMAN-CELLS ON PLASMA POLYMERS - PUTATIVE ROLE OF AMINE AND AMIDE GROUPS
    GRIESSER, HJ
    CHATELIER, RC
    GENGENBACH, TR
    JOHNSON, G
    STEELE, JG
    [J]. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 1994, 5 (06) : 531 - 554