Preparation of artificial metalloenzymes by insertion of chromium(III) Schiff base complexes into apomyoglobin mutants

被引：186

作者：

Ohashi, M

Koshiyama, T

Ueno, T

Yanase, M

Fujii, H

Watanabe, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Dept Chem, Nagoya, Aichi 4648602, Japan

[2] Grad Univ Adv Studies, Dept Struct Mol Sci, Okazaki, Aichi 4448585, Japan

[3] Nagoya Univ, Res Ctr Mat Sci, Nagoya, Aichi 4648602, Japan

[4] Inst Mol Sci, Okazaki, Aichi 4448585, Japan

[5] Ctr Integrat Biosci, Okazaki, Aichi 4448585, Japan

[6] Inst Mol Sci, Okazaki, Aichi 4448585, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 09期

关键词：

D O I：

10.1002/anie.200390256

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Insertion of a symmetric metal complex, [CrIII(5,5′-tBu-salophen)]+ (H2salophen = N,N′-bis(salicylidene)-1,2-phenylenediamine), into the active site of apomyoglobin is demonstrated (see picture). The metal ion and the ligand structure are very important factors that influence the binding affinity of the metal complex with the myoglobin (Mb) cavity. Semisynthetic metalloenzymes can catalyze enantioselective sulfoxidation by using the chiral protein cavity.

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页码：1005 / +

页数：5

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