Host range of Kaposi's sarcoma-associated herpesvirus in cultured cells

被引:190
作者
Bechtel, JT
Liang, YY
Hvidding, J
Ganem, D
机构
[1] Univ Calif San Francisco, Med Ctr, Howard Hughes Med Inst, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Med Ctr, Dept Med, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Med Ctr, Dept Microbiol, San Francisco, CA 94143 USA
关键词
D O I
10.1128/JVI.77.11.6474-6481.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Difficulties in efficiently propagating Kaposi's sarcoma-associated herpesvirus (KSHV) in culture have generated the impression that the virus displays a narrow host range. Here we show that, contrary to expectation, KSHV can establish latent infection in many adherent cell lines, including human and nonhuman cells of epithelial, endothelial, and mesenchymal origin. (Paradoxically, the only lines in which we have not observed successful latent infection are cultured lymphoma cell lines.) In most latently infected lines, spontaneous lytic replication is rare and (with only two exceptions) is not efficiently induced by phorbol ester treatment-a result that explains the failure of most earlier studies to observe efficient serial transfer of infection. However, ectopic expression of the KSHV lytic switch protein RTA from an adenoviral vector leads to the prompt induction of lytic replication in all latently infected lines, with the production of infectious KSHV virions. These results indicate (i) that the host cell receptor(s) and entry machinery for KSHV are widely distributed on cultured adherent cells, (ii) that latency is the default pathway of infection, and (iii) that blocks to lytic induction are frequent and largely reside at or upstream of the expression of KSHV RTA.
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页码:6474 / 6481
页数:8
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