Replication of the linear chromosomal DNA from the centrally located oriC of Streptomyces ambofaciens revealed by PFGE gene dosage analysis

被引：8

作者：

Fischer, G

Holl, AC

Volff, JN

Vandewiele, D

Decaris, B

Leblond, P

机构：

[1] Univ Nancy 1, Fac Sci, Lab Genet & Microbiol, Unite Associee INRA 952, F-54506 Vandoeuvre Nancy, France

[2] Univ Sci & Tech Lille Flandres Artois, Lab Genet & Evolut Populat, F-59655 Villeneuve Dascq, France

[3] Univ Wurzburg, Theodor Boveri Inst Biosci, Bioctr, D-97097 Wurzburg, Germany

[4] NICHD, NIH, Bethesda, MD 20892 USA

来源：

RESEARCH IN MICROBIOLOGY | 1998年 / 149卷 / 03期

关键词：

Streptomyces ambofaciens; oriC; linear chromosome; replication; PFGE;

D O I：

10.1016/S0923-2508(98)80080-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

From a cosmid clone of Streptomyces ambofaciens containing the dnaA and gyrAB genes, a 2.7-kb self-replicating DNA fragment containing the chromosome replication origin oriC was isolated. This cosmid was previously mapped physically to a region near the middle of the 8-Mb linear chromosomal DNA. A pulsed-field gel electrophoresis time-course analysis revealed that sequences flanking oriC were overrepresented relative to the rest of the chromosomal DNA during rapid growth, indicating that this origin is active. In addition, the terminal regions of the chromosomal DNA showed a slight overrepresentation at the onset of stationary phase.

引用

页码：203 / 210

页数：8

共 31 条

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