Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus

被引：95

作者：

DiRuggiero, J ^{[1
]}

Santangelo, N ^{[1
]}

Nackerdien, Z ^{[1
]}

Ravel, J ^{[1
]}

Robb, FT ^{[1
]}

机构：

[1] NIST, DIV BIOTECHNOL, GAITHERSBURG, MD 20899 USA

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 14期

关键词：

D O I：

10.1128/jb.179.14.4643-4645.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of Co-60 gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented: into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95 degrees C. We suggest that recombination repair could be an extremely active repair mechanism in P. furiosus and that it might be an important determinant of survival of hyperthermophiles at high temperatures.

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页码：4643 / 4645

页数：3

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