Carbon source-dependent transcriptional regulation of the mitochondrial glycerol-3-phosphate dehydrogenase gene, GUT2, from Saccharomyces cerevisiae

被引：36

作者：

Grauslund, M ^{[1
]}

Ronnow, B ^{[1
]}

机构：

[1] Danisco Cultor Innovat, DK-1001 Copenhagen K, Denmark

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2000年 / 46卷 / 12期

关键词：

GUT2; mitochondrial glycerol-3-phosphate dehydrogenase; transcriptional regulation; Saccharomyces cerevisiae;

D O I：

10.1139/cjm-46-12-1096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosolic glycerol kinase (Gut1p) and mitochondrial glycerol-3-phosphate dehydrogenase (Gut2p) constitute the glycerol utilization pathway in Saccharomyces cerevisiae. Transcriptional analysis of the GUT2 gene showed that it was repressed by glucose and derepressed on the non-fermentable carbon sources, glycerol, lactate and ethanol. Derepression of GUT2 requires the protein kinase Snf1p as well as the heteromeric protein complex, Hap2/3/4/5, and its putative DNA-binding site (UAS(HAP)) located in the promoter region. Furthermore, glucose repression of GUT2 requires the negative regulator, Opi1p.

引用

页码：1096 / 1100

页数：5

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