Abscisic acid-induced phosphoinositide turnover in guard cell protoplasts of Vicia faba

被引:138
作者
Lee, YS
Choi, YB
Suh, S
Lee, J
Assmann, SM
Joe, CO
Kelleher, JF
Crain, RC
机构
[1] UNIV CONNECTICUT, DEPT MOLEC & CELL BIOL, STORRS, CT 06269 USA
[2] POHANG UNIV SCI & TECHNOL, DEPT LIFE SCI, POHANG 790600, SOUTH KOREA
[3] PENN STATE UNIV, DEPT BIOL, UNIVERSITY PK, PA 16802 USA
[4] KOREA ADV INST SCI & TECHNOL, DEPT LIFE SCI, TAEJON 305701, SOUTH KOREA
关键词
D O I
10.1104/pp.110.3.987
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Guard cell protoplasts of Vicia faba treated with 10 mu M (+)abscisic acid (ABA) in the light exhibited a 20% decrease in diameter within 1.5 h, from 24.1 to 19.6 mu m. Within 10 s of administration of ABA, a 90% increase in levels of inositol 1,4,5-trisphosphate was observed, provided that cells were treated with Li+, an inhibitor of inositol phosphatase activity, prior to incubation. Concomitantly, levels of P-32-labeled phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate decreased 20% compared to levels in control cells; levels of label in the membrane lipids phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol did not change significantly in response to ABA treatment. These results show that phosphoinositide turnover is activated in response to ABA in guard cells. We conclude that phosphoinositide signaling is likely to be a step in the biochemical cascade that couples ABA to guard cell shrinking and stomatal closure.
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页码:987 / 996
页数:10
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