Modulation of cell cycle-specific gene expressions at the onset of S phase arrest contributes to the robust DNA replication checkpoint response in fission yeast

被引:27
作者
Chu, Zhaoqing
Li, Juntao
Eshaghi, Majid
Peng, Xu
Karuturi, R. Krishna M.
Liu, Jianhua [1 ]
机构
[1] Genome Inst Singapore, Singapore 118672, Singapore
[2] Natl Univ Singapore, Dept Biochem, Singapore 117595, Singapore
关键词
D O I
10.1091/mbc.E06-10-0928
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fission yeast replication checkpoint kinases Rad3p and Cds1p are essential for maintaining cell viability after transient treatment with hydroxyurea (HU), an agent that blocks DNA replication. Although current studies have focused on the cyclin-dependent protein kinase Cdc2p that is regulated by these checkpoint kinases, other aspects of their functions at the onset of S phase arrest have not been fully understood. In this study, we use genome-wide DNA microarray analyses to show that HU-induced change of expression profiles in synchronized G(2) cells occurs specifically at the onset of S phase arrest. Induction of many core environmental stress response genes and repression of ribosomal genes happen during S phase arrest. Significantly, peak expression level of the MluI-like cell cycle box (MCB)-cluster (G,) genes is maintained at the onset of S phase arrest in a Rad3p- and Cds1p-dependent manner. Expression level maintenance of the MCB-cluster is mediated through the accumulation of Rep2p, a putative transcriptional activator of the MBF complex. Conversely, the FKH-cluster (M) genes are repressed during the onset of S phase arrest in a Rad3p-dependent manner. Repression of the FKH-cluster genes is mediated through the decreased levels of one of the putative forkhead transcription factors, Sep1p, but not Fkh2p. Together, our results demonstrate that Rad3p and Cds1p modulate transcriptional response during the onset of S phase arrest.
引用
收藏
页码:1756 / 1767
页数:12
相关论文
共 49 条
[1]   IDENTIFICATION AND CHARACTERIZATION OF NEW ELEMENTS INVOLVED IN CHECKPOINT AND FEEDBACK CONTROLS IN FISSION YEAST [J].
ALKHODAIRY, F ;
FOTOU, E ;
SHELDRICK, KS ;
GRIFFITHS, DJF ;
LEHMANN, AR ;
CARR, AM .
MOLECULAR BIOLOGY OF THE CELL, 1994, 5 (02) :147-160
[2]   Feedback regulation of the MBF transcription factor by cyclin Cig2 [J].
Ayté, J ;
Schweitzer, C ;
Zarzov, P ;
Nurse, P ;
DeCaprio, JA .
NATURE CELL BIOLOGY, 2001, 3 (12) :1043-1050
[3]  
Bähler J, 1998, YEAST, V14, P943, DOI 10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO
[4]  
2-Y
[5]   Control of S-phase periodic transcription in the fission yeast mitotic cycle [J].
Baum, B ;
Wuarin, J ;
Nurse, P .
EMBO JOURNAL, 1997, 16 (15) :4676-4688
[6]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[7]   Systematic deletion analysis of fission yeast protein kinases [J].
Bimbó, A ;
Jia, YH ;
Poh, SL ;
Karuturi, RKM ;
den Elzen, N ;
Peng, X ;
Zheng, LL ;
O'Connell, M ;
Liu, ET ;
Balasubramanian, MK ;
Liu, JH .
EUKARYOTIC CELL, 2005, 4 (04) :799-813
[8]   Replication checkpoint enforced by kinases Cds1 and Chk1 [J].
Boddy, MN ;
Furnari, B ;
Mondesert, O ;
Russell, P .
SCIENCE, 1998, 280 (5365) :909-912
[9]  
Brondello JM, 1999, MOL CELL BIOL, V19, P4262
[10]   Fkh2p and Sep1p regulate mitotic gene transcription in fission yeast [J].
Buck, V ;
Ng, SS ;
Ruiz-Garcia, AB ;
Papadopoulou, K ;
Bhatti, S ;
Samuel, JM ;
Anderson, M ;
Millar, JBA ;
McInerny, CJ .
JOURNAL OF CELL SCIENCE, 2004, 117 (23) :5623-5632