Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP)

被引：15

作者：

Asaeda, A

Ide, H

Tano, K

Takamori, Y

Kubo, K ^{[1
]}

机构：

[1] Univ Osaka Prefecture, Coll Agr, Sch Vet Med, Dept Vet Radiol, Osaka 593, Japan

[2] Hiroshima Univ, Fac Sci, Grad Dept Gene Sci, Higashihiroshima 739, Japan

[3] Kyoto Univ, Inst Res Reactor, Kumatori, Osaka 59004, Japan

来源：

NUCLEOSIDES & NUCLEOTIDES | 1998年 / 17卷 / 1-3期

关键词：

D O I：

10.1080/07328319808005194

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human methylpurine N-glycosylase (MPG) activity was investigated by monitoring abasic (AP) sites resulting from removal of alkylated bases. The amount of AP sites in MMS-treated HeLa cells transiently increased at 3 h, then gradually decreased to 40% at 24 h. The presence of adenine, an inhibitor of AP endonucleases, in the repair incubation of MMS-treated cells induced moderate accumulation of AP sites, suggesting inhibition of the activities of MPG as well as AP endonucleases by adenine metabolites.

引用

收藏

页码：503 / 513

页数：11

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