GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K+-selective, K+-sensing ion channel

被引：273

作者：

Ache, P ^{[1
]}

Becker, D ^{[1
]}

Ivashikina, N ^{[1
]}

Dietrich, P ^{[1
]}

Roelfsema, MRG ^{[1
]}

Hedrich, R ^{[1
]}

机构：

[1] Univ Wurzburg, Julius von Sachs Inst Biowissensch, Lehrstuhl Mol Pflanzenphysiol & Biophys, D-97082 Wurzburg, Germany

来源：

FEBS LETTERS | 2000年 / 486卷 / 02期

关键词：

potassium transport; guard cell; Arabidopsis thaliana;

D O I：

10.1016/S0014-5793(00)02248-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report on the molecular identification, guard cell expression and functional characterization of AtGORK, an Arabidopsis thaliana guard cell outward rectifying K+-channel. GORK represents a new member of the plant Shaker K+ channel superfamily.When heterologously expressed in Xenopus oocytes the gene product of GORK mediated depolarization-activated K+ currents. In agreement with the delayed outward rectifier in intact guard cells and protoplasts thereof, GORK is activated in a voltage- and potassium-dependent manner. Furthermore, the single channel conductance and regulation of GORK in response to pH changes resembles the biophysical properties of the guard cell delayed outward rectifier. Thus GORK very likely represents the molecular entity for depolarization-induced potassium release from guard cells. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：93 / 98

页数：6

共 25 条

[1] FUNCTIONAL EXPRESSION OF A PROBABLE ARABIDOPSIS-THALIANA POTASSIUM CHANNEL IN SACCHAROMYCES-CEREVISIAE [J].