The nuclear export receptor XPO-1 supports primary miRNA processing in C. elegans and Drosophila

被引：58

作者：

Buessing, Ingo ^{[1
]}

Yang, Jr-Shiuan ^{[2
]}

Lai, Eric C. ^{[2
]}

Grosshans, Helge ^{[1
]}

机构：

[1] Friedrich Miescher Inst Biomed Res, CH-4002 Basel, Switzerland

[2] Sloan Kettering Inst, Dept Dev Biol, Rockefeller Res Labs, New York, NY USA

来源：

EMBO JOURNAL | 2010年 / 29卷 / 11期

基金：

瑞士国家科学基金会;

关键词：

CBP20/NCBP-2; CBP80/NCBP-1; microRNA biogenesis; nuclear export; XPO1/CRM1/Embargoed; CAP-BINDING COMPLEX; CAENORHABDITIS-ELEGANS; RNA-INTERFERENCE; MICROPROCESSOR COMPLEX; CELL-DIFFERENTIATION; MICRORNA PRECURSORS; PROTEIN; GENES; GW182; CRM1;

D O I：

10.1038/emboj.2010.82

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNA (miRNA) biogenesis proceeds from a primary transcript (pri- miRNA) through the pre-miRNA into the mature miRNA. Here, we identify a role of the Caenorhabditis elegans nuclear export receptor XPO-1 and the cap-binding proteins CBP-20/NCBP-2 and CBP80/NCBP-1 in this process. The RNA-mediated interference of any of these genes causes retarded heterochronic phenotypes similar to those observed for animals with mutations in the let-7 miRNA or core miRNA machinery genes. Moreover, pre- and mature miRNAs become depleted, whereas primary miRNA transcripts accumulate. An involvement of XPO-1 in miRNA biogenesis is conserved in Drosophila, in which knockdown of Embargoed/XPO-1 or its chemical inhibition through leptomycin B causes pri-miRNA accumulation. Our findings demonstrate that XPO-1/Emb promotes the pri-miRNA-to-pre-miRNA processing and we propose that this function involves intranuclear transport and/or nuclear export of primary miRNAs. The EMBO Journal (2010) 29, 1830-1839. doi:10.1038/emboj.2010.82; Published online 30 April 2010

引用

页码：1830 / 1839

页数：10

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